Although phosphine resistance monitoring and management programs are well advanced in Australia, the tropical northern part of the continent has received little attention. To address this, grain storages were sampled in the Townsville region in the Burdekin river catchment area of northern Queensland, and insect populations were subjected to resistance testing. Sampling of storages including silos, food processing facilities and feedlots was carried out during July to August 2019 and February to March 2020. Of the several species collected, populations of two major pest species, Rhyzopertha dominica and Tribolium castaneum were subjected to phenotypic resistance and molecular screening using discriminating doses and gene specific DNA marker, respectively. Utilising phenotypic assay, of the 17 populations of R. dominica, 12 were diagnosed as weakly resistant and five as strongly resistant; whereas of the 34 populations of T. castaneum, two were found to be susceptible to phosphine, 15 were determined as weakly resistant and 17 as strongly resistant. The molecular diagnostics, however, determined rph2 alleles for strong resistance in some populations each of R. dominica and T. castaneum that were diagnosed as weakly resistant in the phenotypic assay. The most prevalent rph2 alleles were found to be P49S and K142E in R. dominica populations and P45S and G131S in T. castaneum populations. Our results highlight the need of more comprehensive study towards determining the level of pests and resistance risks in this region.

尽管在澳大利亚，耐膦化氢的监测和管理计划已经很完善，但该大陆的热带北部却鲜有受到关注。为了解决这个问题，在昆士兰州北部的伯德金河集水区的汤斯维尔地区对谷物仓库进行了采样，并对昆虫种群进行了抗性测试。在2019年7月至2019年8月和2020年2月至2020年3月期间对包括筒仓，食品加工设施和饲养场的储存进行了采样。在所收集的几种物种中，有两种主要害虫物种，即Rhyzopertha dominica和Tribolium castaneum的种群。分别使用区分剂量和基因特异性DNA标记物进行表型抗性和分子筛选。利用表型分析，在多米尼加的17个种群中，有12个被诊断为弱抗性，有5个为强抗性。然而，在34个cast树种群中，发现2个对磷化氢敏感，其中15个被确定为弱抗性，而17个被确定为强抗性。在分子诊断，然而，确定rph2等位基因在每个的某些人群强阻力R.蠹和赤拟谷盗的是被诊断为在表型分析弱抗性。最流行的rph2等位基因被发现在多米尼加（R. dominica）种群中为P49S和K142E，在锥栗木（T. castaneum）种群中为P45S和G131S 。我们的结果强调需要进行更全面的研究，以确定该区域的有害生物水平和抗药性风险。