Cystic Echinococcosis (CE) is a parasitic infection caused by the larval stage of Echinococcus granulosus. Exploring safe and effective scolicidal agents for the surgery is an urgent need for the successful treatment of CE. This study aimed to determine scolicidal activity of the synthesized chitosan nanoparticles. Physicochemical properties of synthesized nanoparticles were determined by using DLS, FTIR, and SEM. Different concentrations of chitosan nanoparticles from 125 to 1000 μg/ml were examined at different incubation times (10, 60, 120, and 180 min). Scolicidal and cytotoxic activity of chitosan nanoparticles were confirmed by eosin exclusion and hemolysis activity tests. FTIR spectra, zeta potential (+42 ± 2.08) and PDI (0.388 ± 0.034) value revealed that the chitosan nanoparticles were synthesized. Significant differences among the scolicidal effects of chitosan nanoparticles were observed in comparison to the control treatments and highest scolicidal activity was observed at 1000 μg/ml after 180 min exposure time. Hemolytic activity was not significant at all concentrations of chitosan nanoparticles. Our findings support the hypothesis that Chitosan nanoparticles have the potential to be a safe and efficient scolicidal agent candidate at very low concentrations and in a wide range of exposure time. Further in vivo studies are recommended to evaluate chitosan nanoparticle efficacy before clinical application.

囊性棘球co虫病（CE）是由细粒棘球oc虫的幼虫期引起的寄生虫感染。为了成功地治疗CE，迫切需要探索一种安全有效的手术杀伤剂。这项研究旨在确定合成的壳聚糖纳米颗粒的杀卵活性。合成的纳米粒子的理化性质通过DLS，FTIR和SEM确定。在不同的孵育时间（10、60、120和180分钟）下检测了125至1000μg/ ml的不同浓度的壳聚糖纳米颗粒。曙红排除和溶血活性测试证实了壳聚糖纳米颗粒的杀伤和细胞毒性活性。FTIR光谱，ζ电位（+42±2.08）和PDI（0.388±0.034）值表明已合成了壳聚糖纳米颗粒。与对照处理相比，壳聚糖纳米颗粒的杀卵作用之间存在显着差异，暴露180分钟后，在1000μg/ ml的条件下观察到了最高的杀卵活性。在所有浓度的壳聚糖纳米颗粒上，溶血活性均不显着。我们的发现支持以下假设，即壳聚糖纳米粒子在非常低的浓度下和宽范围的暴露时间内有可能成为安全有效的杀伤剂的候选者。建议在临床应用之前进行进一步的体内研究，以评估壳聚糖纳米颗粒的功效。我们的发现支持以下假设，即壳聚糖纳米粒子在非常低的浓度下和宽范围的暴露时间内有可能成为安全有效的杀伤剂的候选者。建议在临床应用之前进行进一步的体内研究，以评估壳聚糖纳米颗粒的功效。我们的发现支持以下假设，即壳聚糖纳米粒子在非常低的浓度下和宽范围的暴露时间内有可能成为安全有效的杀伤剂的候选者。建议在临床应用之前进行进一步的体内研究，以评估壳聚糖纳米颗粒的功效。