Objectives

With generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is the fastest growing non-pathogenic bacterium isolated so far. The availability of the full genome and genetic engineering tools and its ability to utilize a wide range of carbon sources make V. natriegens an attractive host for biotechnological production processes. However, high-cell-density cultivations, which are desired at industrial-scale have not been described so far.

Results

In this study we report fed-batch cultivations of V. natriegens in deep-well plates and lab-scale bioreactor cultivations at different temperatures in mineral salt medium (MSM). Upon switching from exponential glucose to constant glucose-feeding cell death was induced. Initial NaCl concentrations of 15–18 g L⁻¹ and a temperature reduction from 37 to 30 °C had a positive effect on cell growth. The maximal growth rate in MSM with glucose was 1.36 h⁻¹ with a specific oxygen uptake rate of 22 mmol g_CDW⁻¹ h⁻¹. High biomass yields of up to 55 g L⁻¹ after only 12 h were reached.

Conclusions

The shown fed-batch strategies demonstrate the potential of V. natriegens as a strong producer in industrial biotechnology.

中文翻译：

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Vibrio natriegens 的高细胞密度补料分批培养

目标

在最佳条件下，产生时间不到 10 分钟，嗜盐弧菌是迄今为止分离出的生长最快的非致病菌。全基因组和基因工程工具的可用性及其利用广泛碳源的能力使V. natriegens成为生物技术生产过程的有吸引力的宿主。然而，目前尚未描述工业规模所需的高细胞密度培养。

结果

在这项研究中，我们报告了深孔板中V. natriegens的分批补料培养和在不同温度下在矿物盐培养基 (MSM) 中的实验室规模生物反应器培养。在从指数葡萄糖转换为恒定的葡萄糖喂养细胞死亡时被诱导。^{15-18 g L -1}的初始 NaCl 浓度和从 37 到 30 °C 的温度降低对细胞生长有积极影响。在 MSM 中，葡萄糖的最大生长速率为 1.36 h ^-1，特定的摄氧速率为 22 mmol g _CDW ^-1 h ^-1。仅在 12 小时后就达到了高达 55 g L ^{-1的高生物质产量。}

结论

所示的分批补料策略展示了V. natriegens作为工业生物技术强大生产者的潜力。