Nanoparticle Tracking Analysis of Polymer Nanoparticles in Blood Plasma,Particle & Particle Systems Characterization

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Nanoparticle Tracking Analysis of Polymer Nanoparticles in Blood Plasma
Particle & Particle Systems Characterization ( IF 2.7 ) Pub Date : 2021-05-19 , DOI: 10.1002/ppsc.202100016
Mark S. Bannon _{1,

2} , Aida López Ruiz ₁ , Karen Corrotea Reyes ₁ , Miriam Marquez ₁ , Zahra Wallizadeh ₁ , Mohammad Savarmand ₁ , Connor A. LaPres ₃ , Joerg Lahann _{3,

4,

5} , Kathleen McEnnis _{1,

4}

Affiliation

A successful drug delivery system must overcome complex biological barriers. For particles injected into the blood, one of the first and most critical barriers pertains to blood stability to circulate through the human body. To effectively design drug delivery vehicles, interactions between the particles and blood, as well as the aggregation behavior, must be understood. This work presents a method to analyze particle size and aggregation in blood plasma using a commercially available nanoparticle tracking analysis (NTA) system. As a model system, fluorescently labeled polystyrene nanoparticles are incubated in goat blood plasma and analyzed using NTA. The particles incubated in plasma are found to have a protein corona that is larger than what has been observed by dynamic light scattering (DLS) in diluted plasma. Particles that are decorated with a PEG layer are also found to have large protein coronas in undiluted plasma. Because NTA is based on a unique visualization method, large multicomponent aggregates could be observed and quantified in a manner not feasible with other techniques. PEGylation of the particles is found to decrease the multicomponent aggregation from 1000 ± 200 particles for unmodified to 200 ± 30 particles for 1K PEGylated per 1 × 10⁵ total particles.

中文翻译：

血浆中聚合物纳米粒子的纳米粒子追踪分析

成功的给药系统必须克服复杂的生物障碍。对于注射到血液中的颗粒，第一个也是最关键的障碍之一与血液在人体中循环的稳定性有关。为了有效地设计药物输送载体，必须了解颗粒与血液之间的相互作用以及聚集行为。这项工作提出了一种使用市售纳米颗粒跟踪分析 (NTA) 系统分析血浆中颗粒大小和聚集的方法。作为模型系统，荧光标记的聚苯乙烯纳米粒子在山羊血浆中孵育并使用 NTA 进行分析。发现在血浆中培养的颗粒具有比在稀释的血浆中通过动态光散射 (DLS) 观察到的更大的蛋白冠。还发现用 PEG 层装饰的粒子在未稀释的血浆中具有大的蛋白质冠。由于 NTA 基于独特的可视化方法，因此可以以其他技术不可行的方式观察和量化大型多组分聚集体。发现颗粒的聚乙二醇化将多组分聚集从未修饰的 1000 ± 200 个颗粒减少到每 1 × 10 1K 聚乙二醇化的 200 ± 30 个颗粒共⁵个粒子。

更新日期：2021-06-20

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