Multifunctional nanodrugs have emerged as an effective platform to integrate multiple imaging and therapeutic functions for tremendous biomedical applications. However, the development of a simple potent theranostic nanoplatform is still an intractable challenge. Herein, a novel theranostic nanoplatform was developed by coupling prepared Au nanobipyramids with Gd₂O₃, Au nanoclusters and denatured bovine serum albumin (AuNBP-Gd₂O₃/Au-dBSA) for FL/MR dual-modal imaging guided photothermal therapy. AS1411 aptamers were conjugated to enhance its targetability towards breast cancer. The AS1411-AuNBP-Gd₂O₃/Au-dBSA suspension could be readily heated above 40 °C at a low concentration (2 mg/L) and NIR density (1 W/cm²). The AS1411-AuNBP-Gd₂O₃/Au-dBSA revealed a fluorescence quantum yield of 4.2% and higher longitudinal relaxivity rate of 6.75 mM⁻¹ s⁻¹ compared to Gd-DTPA of 4.45 mM⁻¹ s⁻¹. As a result, the AS1411-AuNBP-Gd₂O₃/Au-dBSA functions as a multimodal nanoprobe of photothermal, fluorescence and MR imaging for specific tumor diagnosis and guidance of therapy, which was validated via in vitro and in vivo tests. Moreover, AS1411-AuNBP-Gd₂O₃/Au-dBSA nanoparticles indicated excellent photothermal anticancer effect more than 95% in both in vitro and in vivo tests. Besides, the low toxicity of AS1411-AuNBP-Gd₂O₃/Au-dBSA nanocomposites was further confirmed in vitro and in vivo. Thus, these results demonstrated the AS1411-AuNBP-Gd₂O₃/Au-dBSA nanocomposites as a rational design of multifunctional nanoplatform to enable multimodal imaging guided photothermal therapy.

多功能纳米药物已成为整合多种成像和治疗功能以实现巨大生物医学应用的有效平台。然而，开发简单有效的治疗药物纳米平台仍然是棘手的挑战。在本文中，通过将制备的Au纳米双金字塔与Gd ₂ O ₃，Au纳米簇和变性的牛血清白蛋白（AuNBP-Gd ₂ O ₃ / Au-dBSA）偶联用于FL / MR双模态成像引导光热疗法，开发了一种新型的治疗纳米平台。共轭AS1411适体可增强其对乳腺癌的靶向性。AS1411-AuNBP-Gd ₂ O ₃/ Au-dBSA悬浮液可以很容易地以低浓度（2 mg / L）和NIR密度（1 W / cm ²）加热到40°C以上。的AS1411-AuNBP的Gd ₂ ö ₃ / Au的DBSA揭示的4.2％荧光量子产率和更高的纵向弛豫率6.75毫^-1 小号^-1相比，4.45毫钆DTPA ^-1 小号^-1。结果，AS1411-AuNBP-Gd ₂ O ₃ / Au-dBSA可以作为光热，荧光和MR成像的多峰纳米探针，用于特定的肿瘤诊断和治疗指导，并已通过体内和体外测试进行了验证。此外，AS1411-AuNBP-Gd ₂在体外和体内试验中，O ₃ / Au-dBSA纳米颗粒均显示出超过95％的优异光热抗癌效果。此外，在体外和体内进一步证实了AS1411-AuNBP-Gd ₂ O ₃ / Au-dBSA纳米复合材料的低毒性。因此，这些结果证明了AS1411-AuNBP-Gd ₂ O ₃ / Au-dBSA纳米复合材料是多功能纳米平台的合理设计，能够实现多模态成像引导的光热疗法。