The objective of this study was to characterize antimicrobial resistance (AMR) of WHO priority 1 critical pathogen (extrapathogenic Escherichia coli (ExPEC), sequence types (STs), and ST131 clades from patients in Tanzania so as to guide specific antimicrobial therapies and preventive measures. A total of 143 ExPEC strains (128 from pregnant women with urinary tract infections and 15 from children with blood stream infections) were collected between March 2016 and October 2017. These were characterized into ST-fimH clones by a 7-single nucleotide polymorphism quantitative polymerase chain reaction (7-SNP qPCR) and gene sequencing, and to ST131 clades by multiplex PCR. The extended-spectrum beta-lactamases (ESBL) production was 16.1% (23/143), and was predominantly due to the bla_CTX-M-15 (91.3%, n=21). ESBL production was significantly more among strains from children (53.3%) than pregnant women (11.7%) (OR (95%CI): 8.61 (2.73–27.15); p-value <0.001)). Approximately 61.5% (n=88) ExPEC were typed into their respective STs/CCs (87 by the 7-SNP qPCR and by an additional of one or two genes sequencing). The commonest STs/CCs among typeable strains were CC10 (28.4%, n=25), ST131 (18.2%, n=16), and ST38 (10.2%, n=9). The ST131 clades (C1 (4, 25.0%) and C2 (6, 37.5%)) were predominantly associated with fluoroquinolone resistance and ESBL production, respectively. Approximately 60.8% of ExPEC strains and all dominant clones were typed by the 7-SNP qPCR by additional sequencing. The multiplex clade PCR allowed linkage of the global clone ST131 with AMR phenotypes. These feasible and user-friendly molecular tools can be routinely used for surveillance programs in resource-limited settings.

这项研究的目的是表征世界卫生组织优先级1关键病原体（超病原性大肠杆菌（ExPEC），序列类型（STs）和来自坦桑尼亚患者的ST131进化枝）的抗药性（AMR），以指导具体的抗微生物治疗和预防措施在2016年3月至2017年10月之间，共收集了143株ExPEC菌株（128例来自尿路感染的孕妇，15例来自血流感染的儿童），并通过7个单核苷酸多态性定量分析鉴定为ST- fimH克隆。聚合酶链反应（7-SNP qPCR）和基因测序，并通过多重PCR扩增到ST131进化枝，扩大光谱的β-内酰胺酶（ESBL）产生率为16.1％（23/143），主要是由于bla _CTX-M-15（91.3％，n = 21）。儿童菌株中的ESBL产量（53.3％）比孕妇（11.7％）显着多（OR（95％CI）：8.61（2.73–27.15）； p值<0.001））。将大约61.5％（n = 88）的ExPEC输入其各自的ST / CC中（通过7-SNP qPCR和另外一个或两个基因测序，将其分类为87）。在可分型菌株中最常见的STs / CCs是CC10（28.4％，n = 25），ST131（18.2％，n = 16）和ST38（10.2％，n = 9）。ST131进化枝（C1（4，25.0％）和C2（6，37.5％））主要分别与氟喹诺酮耐药性和ESBL产生有关。通过额外的测序，通过7-SNP qPCR对大约60.8％的ExPEC菌株和所有优势克隆进行分型。多重进化枝PCR允许整体克隆ST131与AMR表型连接。