Finding novel and efficient compounds to be used against cancers is a global research concern. Conjugation of magnetic compounds to silver nanoparticles (NPs) could provide novel characteristics for the nanocomposite. In this work, TiFe₂O₄@Ag NPs were synthesized, and their physicochemical properties were investigated using FT-IR, XRD, EDX, Zeta potential, VSM, DLS, and electron microscopy analyses. Finally, anticancer potential of the NPs was evaluated using the cellular and molecular assays. The results showed the proper synthesis of the spherical NPs with the size range of 20–60 nm. The nanocomposite had good stability, with the zeta potential of -47.7 mV, and lack of elemental impurities. The MTT assay revealed that the TiFe₂O₄@Ag NPs were significantly more toxic toward the AGS gastric cancer cells (IC₅₀ = 69.6 µg/mL), than normal HEK293 cells (IC₅₀ = 130 µg/mL). Flow cytometry analysis showed apoptosis induction among NPs treated cells than controls with the frequency of 12.1 and 9.82% for the early and late apoptosis, respectively. In agreement with the flow cytometry and MTT assays, we found that the activity of caspase-3 increased to 1.81 folds, compared with the control cells. Also, the expression of the Bax and p53 genes was increased by 1.4 and 1.6 folds, while the Bcl-2 gene was reduced to 0.7 folds, in comparison with the controls. Our study presented TiFe₂O₄@Ag NPs as novel and promising anticancer agent which need further characterization to be used in biomedical applications.

寻找用于癌症的新颖有效的化合物是全球研究的关注点。磁性化合物与银纳米颗粒（NPs）的共轭可以为纳米复合材料提供新颖的特性。在这项工作中，合成了TiFe ₂ O ₄ @Ag NP，并使用FT-IR，XRD，EDX，Zeta电位，VSM，DLS和电子显微镜分析研究了它们的理化性质。最后，使用细胞和分子测定法评估了NPs的抗癌潜力。结果表明，球形NP的合成正确，尺寸范围为20-60 nm。该纳米复合材料具有良好的稳定性，其ζ电位为-47.7 mV，并且没有元素杂质。MTT分析表明，TiFe ₂ O ₄ 与正常的HEK293细胞（IC ₅₀  = 130 µg / mL）相比，@ Ag NPs对AGS胃癌细胞的毒性（IC ₅₀ = 69.6 µg / mL）明显更高。流式细胞仪分析显示，与对照组相比，NPs处理的细胞凋亡诱导率更高，早期和晚期凋亡的发生率分别为12.1和9.82％。与流式细胞仪和MTT分析一致，我们发现caspase-3的活性与对照细胞相比增加了1.81倍。另外，与对照相比，Bax和p53基因的表达增加了1.4和1.6倍，而Bcl-2基因的表达减少了0.7倍。我们的研究提出了TiFe ₂ O ₄@Ag NPs是新型且有希望的抗癌药，需要进一步表征才能用于生物医学应用。