Hypotrichia, one of the most complex and highly differentiated groups in Ciliophora, has been the object of extensive studies, especially in recent years. Nevertheless, methodological difficulties and insufficient faunistic studies have limited our understanding of their biodiversity and phylogeny. In this study, one novel urostylid ciliate, Pseudoholosticha zhaoi nov. gen., nov. spec. and two populations of Anteholosticha monilata (Kahl, 1928) Berger, 2003, type species of the latter genus, are studied using an integrative approach (live observation, protargol impregnation, scanning electron microscopy, and phylogenetic analysis) to provide further insights into the diversity, classification, and phylogeny of this group of ciliates. Pseudoholosticha nov. gen. can be separated from other morphologically similar genera mainly by the absence of buccal and caudal cirri. A key to 12 morphologically similar genera and illustrations of their cirral patterns are provided. The validation of the new genus and new species is supported by both morphological and phylogenetic analyses. The first 18S rRNA gene sequence of A. monilata, with detailed morphological data, provided a reliable clarification of A. monilata populations and corroborated the phylogenetic position of the type species of the polyphyletic genus Anteholosticha.

Hypotrichia 是纤毛纲中最复杂和高度分化的群体之一，一直是广泛研究的对象，尤其是近年来。然而，方法学上的困难和动物学研究的不足限制了我们对其生物多样性和系统发育的理解。在这项研究中，一种新的 urostylid 纤毛虫 Pseudoholosticha zhaoi nov。将军，十一月 规格 和两个Anteholosticha monilata (Kahl, 1928) Berger, 2003，后者属的类型物种，使用综合方法（实时观察、protargol 浸渍、扫描电子显微镜和系统发育分析）进行研究，以提供对多样性的进一步见解这组纤毛虫的分类和系统发育。假全息十一月 将军 可以与其他形态相似的属分开，主要是因为没有颊和尾卷须。提供了 12 个形态相似的属的关键和它们的环形图案的插图。新属和新种的验证得到形态学和系统发育分析的支持。A. monilata的第一个 18S rRNA 基因序列具有详细的形态学数据，为A. monilata种群提供了可靠的澄清，并证实了多系Anteholosticha属的模式种的系统发育位置。