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Effect of Curcumin-Hydroxypropyl- $$\beta$$ β -Cyclodextrin Complex and the Complex Loaded Gelatin Carrageenan Microparticles on the Various Chemical and Biological Properties
Journal of Pharmaceutical Innovation ( IF 2.7 ) Pub Date : 2021-05-19 , DOI: 10.1007/s12247-021-09559-0
Bably Khatun , Munmi Majumder , R. Mukhopadhyay , Rafika Yasmin , Robin Doley , T. K. Maji

Abstract

Curcumin was modified with 2-hydroxypropyl-\(\beta\)-cyclodextrin (HP\(\beta\)CD) to enhance its bioavailability. The modified curcumin was loaded into gelatin-carrageenan microparticles to control the drug release behavior. The different analytical techniques like Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (RS), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) indicated the formation of the samples. The solubility of the modified curcumin was checked visibly and by using UV-VIS spectroscopy & optical microscopy as well. The effect of surfactant on process yield, drug loading & encapsulation efficiency, swelling and drug release from the microparticles was checked. The samples exhibited more swelling and hence drug release was more in basic compared to acidic medium and the percentage increased with increase in time. The modified curcumin, on examining in both breast and lung cancer cell lines, manifested better anticancer activity compared to curcumin as evidenced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, clonogenic assay and apoptosis assay. However, the microparticles didn’t reveal better anticancer activities compared to curcumin and modified curcumin. Further, all the prepared samples were found to be non-toxic to human peripheral blood mononuclear cells (PBMCs) and red blood cells (RBCs).

Graphical Abstract



中文翻译:

姜黄素-羟基丙基-$$ \β$$β-环糊精复合物和负载复合明胶角叉菜胶微粒对各种化学和生物学性质的影响

摘要

姜黄素用2-羟丙基- ((β)\-环糊精(HP \(β))修饰CD)以提高其生物利用度。将修饰的姜黄素上样至明胶-角叉菜胶微粒中,以控制药物释放行为。诸如傅立叶变换红外光谱(FTIR),拉曼光谱(RS),X射线衍射法(XRD),差示扫描量热法(DSC)和扫描电子显微镜(SEM)等不同的分析技术表明了样品的形成。可见地并且通过使用UV-VIS光谱法和光学显微镜检查了改性姜黄素的溶解度。检查了表面活性剂对工艺收率,药物载量和包封效率,溶胀和药物从微粒中释放的影响。与酸性介质相比,样品显示出更多的溶胀,因此碱性药物释放更多,并且百分比随时间增加而增加。经修饰的姜黄素在乳腺癌和肺癌细胞系中均进行检测,与姜黄素相比具有更好的抗癌活性,如3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)分析所证明,克隆形成测定和凋亡测定。然而,与姜黄素和改性姜黄素相比,微粒没有显示出更好的抗癌活性。此外,发现所有制备的样品对人外周血单核细胞(PBMC)和红细胞(RBC)无毒。与姜黄素和改性姜黄素相比,微粒没有显示出更好的抗癌活性。此外,发现所有制备的样品对人外周血单核细胞(PBMC)和红细胞(RBC)无毒。与姜黄素和改性姜黄素相比,微粒没有显示出更好的抗癌活性。此外,发现所有制备的样品对人外周血单核细胞(PBMC)和红细胞(RBC)无毒。

图形概要

更新日期:2021-05-19
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