Abstract

Circular RNAs (circRNAs) are a class of non-coding RNAs that could serve as potential molecular markers for disease diagnosis. However, the role of circRNAs in plasma from new-onset rheumatoid arthritis (RA) has not been extensively investigated. In this study, the expression of hsa-circ0000175 and hsa-circ0044235 in plasma from RA patients, healthy controls (HCs), systemic lupus erythematosus (SLE) patients, osteoarthritis (OA), and undiagnosed arthritis (UA) patients were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Correlation analysis was used to assess the correlation of the two circRNAs and clinical variables of RA. The receiver operating characteristic (ROC) curves were created to evaluate the diagnostic value and multivariate analysis (logistic regression) was performed to analyse the risk factors. We confirmed that hsa-circ0000175 was significantly elevated in plasma from patients with new-onset RA compared with HC and patients with new-onset SLE, but significantly was reduced when compared with OA + UA patients. Hsa-circ0044235 was found to be significantly decreased in plasma from patients with new-onset RA compared with HC and OA + UA patients, but was significantly increased compared with SLE patients. The expression of plasma hsa-circ0000175 in new-onset RA patients was associated with platelet count (PLT), plateletcrit (PCT), and platelet large cell ratio (PLR), the expression of plasma hsa-circ0044235 new-onset RA patients was associated with swollen joint count (SJC), painful joint count (PJC), and disease activity score 28 (DAS28). ROC curve analysis suggested that the combination of hsa-circ0000175 and hsa-circ0044235 has some value in the diagnosis of new-onset RA from HC, patients with SLE and patients with OA + UA. The logistic regression analysis revealed that the expression of hsa-circ0000175 and hsa-circ0044235 in plasma were risk factors for RA. This study suggests that the combination of plasma hsa-circ0000175 and hsa-circ0044235 improves the diagnostic accuracy for new-onset RA. Moreover, the expression levels of plasma hsa-circ0000175 and hsa-circ0044235 were associated with disease activity and severity of RA.

摘要

环状 RNA (circRNA) 是一类非编码 RNA，可作为疾病诊断的潜在分子标记。然而，环状RNA在新发类风湿关节炎（RA）血浆中的作用尚未得到广泛研究。在本研究中，通过定量测定 RA 患者、健康对照 (HC)、系统性红斑狼疮 (SLE) 患者、骨关节炎 (OA) 和未确诊关节炎 (UA) 患者血浆中 hsa-circ0000175 和 hsa-circ0044235 的表达。逆转录聚合酶链反应（qRT-PCR）。相关分析用于评估两种circRNA与RA临床变量的相关性。创建接受者操作特征（ROC）曲线以评估诊断价值，并进行多变量分析（逻辑回归）以分析风险因素。我们证实，与 HC 和新发 SLE 患者相比，新发 RA 患者血浆中的 hsa-circ0000175 显着升高，但与 OA + UA 患者相比显着降低。与 HC 和 OA + UA 患者相比，新发 RA 患者血浆中的 Hsa-circ0044235 显着降低，但与 SLE 患者相比显着升高。新发RA患者血浆hsa-circ0000175的表达与血小板计数(PLT)、血小板比容(PCT)和血小板大细胞比值(PLR)相关，新发RA患者血浆hsa-circ0044235的表达与肿胀关节计数 (SJC)、疼痛关节计数 (PJC) 和疾病活动评分 28 (DAS28)。ROC曲线分析提示hsa-circ0000175和hsa-circ0044235联合对HC、SLE患者和OA+UA患者新发RA的诊断具有一定价值。Logistic回归分析显示血浆中hsa-circ0000175和hsa-circ0044235的表达是RA的危险因素。这项研究表明，血浆 hsa-circ0000175 和 hsa-circ0044235 的组合提高了新发 RA 的诊断准确性。此外，血浆 hsa-circ0000175 和 hsa-circ0044235 的表达水平与疾病活动性和 RA 严重程度相关。这项研究表明，血浆 hsa-circ0000175 和 hsa-circ0044235 的组合提高了新发 RA 的诊断准确性。此外，血浆 hsa-circ0000175 和 hsa-circ0044235 的表达水平与疾病活动性和 RA 严重程度相关。这项研究表明，血浆 hsa-circ0000175 和 hsa-circ0044235 的组合提高了新发 RA 的诊断准确性。此外，血浆 hsa-circ0000175 和 hsa-circ0044235 的表达水平与疾病活动性和 RA 严重程度相关。