Scrub typhus develops after the individual is bitten by a trombiculid mite infected with Orientia tsutsugamushi. Since it has been reported that pneumonia is frequently observed in patients with scrub typhus, we investigated whether intranasal (i.n.) vaccination with the outer membrane protein of O. tsutsugamushi (OMPOT) would induce a protective immunity against O. tsutsugamushi infection. It was particular interest that when mice were infected with O. tsutsugamushi, the bacteria disseminated into the lungs, causing pneumonia. The i.n. vaccination with OMPOT induced IgG responses in serum and bronchoalveolar lavage (BAL) fluid. The anti-O. tsutsugamushi IgA Abs in BAL fluid after the vaccination showed a high correlation of the protection against O. tsutsugamushi. The vaccination induced strong Ag-specific Th1 and Th17 responses in the both spleen and lungs. In conclusion, the current study demonstrated that i.n. vaccination with OMPOT elicited protective immunity against scrub typhus in mouse with O. tsutsugamushi infection causing subsequent pneumonia.

中文翻译：

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东方tsu虫外膜蛋白的鼻内疫苗接种可诱导针对斑疹伤寒的保护性免疫。

灌木斑疹伤寒在个体被m虫东方感染的菱螨咬伤后形成。由于有报道说，在擦洗型斑疹伤寒患者中经常观察到肺炎，因此我们调查了鼻内接种虫的外膜蛋白（OMPOT）是否会诱导针对gam虫感染的保护性免疫。特别令人感兴趣的是，当小鼠感染tsu虫O. tsutsugamushi时，细菌会散布到肺部，引起肺炎。接种OMPOT可以诱导血清和支气管肺泡灌洗（BAL）液中的IgG反应。抗O虫阿布斯的IgA BAL液后接种表现出对保护的高相关性恙虫病东方体。疫苗在脾脏和肺部均诱导强烈的Ag特异性Th1和Th17反应。总而言之，当前的研究表明，接种OMPOT疫苗可引起小鼠。虫O. tsutsugamushi感染，引起随后的肺炎，从而对斑疹伤寒提供保护性免疫力。