Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization,Letters in Applied Microbiology

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Lactic acid bacteria biofilms and their ability to mitigate Escherichia coli O157:H7 surface colonization
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-05-18 , DOI: 10.1111/lam.13509
L Cisneros ₁ , N Cattelan _{2,

3} , M I Villalba ₂ , C Rodriguez ₁ , D O Serra ₄ , O Yantorno ₂ , S Fadda ₁

Affiliation

Lactic acid bacteria (LAB) exert antagonistic activities against diverse microorganisms, including pathogens. In this work, we aimed to investigate the ability of LAB strains isolated from food to produce biofilms and to inhibit growth and surface colonization of Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 at 10°C. The ability of 100 isolated LAB to inhibit EHEC O157:H7 NCTC12900 growth was evaluated in agar diffusion assays. Thirty-seven LAB strains showed strong growth inhibitory effect on EHEC. The highest inhibitory activities corresponded to LAB strains belonging to Lactiplantibacillus plantarum, Pediococcus acidilactici and Pediococcus pentosaceus species. Eighteen out of the 37 strains that showed growth inhibitory effects on EHEC also had the ability to form biofilms on polystyrene surfaces at 10°C and 30°C. Pre-established biofilms on polystyrene of four of these LAB strains were able to reduce significantly surface colonization by EHEC at low temperature (10°C). Among these four strains, Lact. plantarum CRL 1075 not only inhibited EHEC but also was able to grow in the presence of the enteric pathogen. Therefore, this strain proved to be a good candidate for further technological studies oriented to its application in food-processing environments to mitigate undesirable surface contaminations of E. coli.

中文翻译：

乳酸菌生物膜及其减轻大肠杆菌 O157:H7 表面定植的能力

乳酸菌 (LAB) 对包括病原体在内的多种微生物发挥拮抗作用。在这项工作中，我们旨在研究从食物中分离的 LAB 菌株在 10°C 下产生生物膜以及抑制肠出血性大肠杆菌(EHEC) O157:H7生长和表面定植的能力。在琼脂扩散试验中评估了 100 个分离的 LAB 抑制 EHEC O157:H7 NCTC12900 生长的能力。37 个 LAB 菌株对 EHEC 显示出强烈的生长抑制作用。最高的抑制活性对应于属于植物乳杆菌、乳酸片球菌和戊糖片球菌的LAB 菌株物种。在对 EHEC 显示出生长抑制作用的 37 种菌株中，有 18 种还能够在 10°C 和 30°C 下在聚苯乙烯表面形成生物膜。其中四种 LAB 菌株在聚苯乙烯上预先建立的生物膜能够在低温 (10°C) 下显着减少 EHEC 的表面定植。在这四种菌株中，Lact。Plantarum CRL 1075 不仅抑制 EHEC，而且能够在肠道病原体存在的情况下生长。因此，该菌株被证明是面向其在食品加工环境中应用以减轻大肠杆菌表面污染的进一步技术研究的良好候选者。

更新日期：2021-07-18

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