The role of rhizosphere microbiome in supporting plant growth under biotic stress is well documented. Rhizobacteria ward off phytopathogens through various mechanisms including antibiosis. We sought to recover novel antibiotic-producing bacterial strains from soil samples collected from the rhizosphere. Pseudomonas fragi A13BB was recovered as part of this effort, and the whole genome was sequenced to facilitate mining for potential antibiotic-encoding biosynthetic gene clusters. Here, we report the complete genome sequence of P. fragi A13BB obtained from de novo assembly of Illumina MiSeq and GridION reads. The 4.94 Mb genome consists of a single chromosome with a GC content of 59.40%. Genomic features include 4410 CDSs, 102 RNAs, 3 CRISPR arrays, 3 prophage regions, and 37 predicted genomic islands. Two β-lactone biosynthetic gene clusters were identified; besides, metabolic products of these are known to show antibiotic and/or anticancer properties. A siderophore biosynthetic gene cluster was also identified even though P. fragi is considered a non-siderophore producing pseudomonad. Other gene clusters of broad interest identified include those associated with bioremediation, biocontrol, plant growth promotion, or environmental adaptation. This dataset unveils various un−/underexplored metabolic or biosynthetic potential of P. fragi and provides insight into molecular mechanisms underpinning these attributes.

中文翻译：

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从头基因组组装和分析揭示了脆弱假单胞菌 A13BB 的生物合成和代谢潜力

根际微生物组在生物胁迫下支持植物生长的作用已得到充分证明。根际细菌通过包括抗菌在内的各种机制来抵御植物病原体。我们试图从从根际收集的土壤样品中回收新的产生抗生素的细菌菌株。作为这项工作的一部分，回收了脆弱假单胞菌 A13BB，并对整个基因组进行了测序，以促进挖掘潜在的抗生素编码生物合成基因簇。在这里，我们报告了从 Illumina MiSeq 和 GridION 读数的从头组装获得的 P. fragi A13BB 的完整基因组序列。4.94 Mb 基因组由一条 GC 含量为 59.40% 的染色体组成。基因组特征包括 4410 个 CDS、102 个 RNA、3 个 CRISPR 阵列、3 个原噬菌体区域和 37 个预测的基因组岛。鉴定了两个β-内酯生物合成基因簇；此外，已知这些代谢产物显示出抗生素和/或抗癌特性。尽管 P. fragi 被认为是非铁载体产生的假单胞菌，但也鉴定了铁载体生物合成基因簇。确定的其他广泛感兴趣的基因簇包括与生物修复、生物控制、植物生长促进或环境适应相关的基因簇。该数据集揭示了 P. fragi 的各种未/未充分探索的代谢或生物合成潜力，并提供了对支撑这些属性的分子机制的深入了解。fragi 被认为是一种非铁载体产生的假单胞菌。确定的其他广泛感兴趣的基因簇包括与生物修复、生物控制、植物生长促进或环境适应相关的基因簇。该数据集揭示了 P. fragi 的各种未/未充分探索的代谢或生物合成潜力，并提供了对支撑这些属性的分子机制的深入了解。fragi 被认为是一种非铁载体产生的假单胞菌。确定的其他广泛感兴趣的基因簇包括与生物修复、生物控制、植物生长促进或环境适应相关的基因簇。该数据集揭示了 P. fragi 的各种未/未充分探索的代谢或生物合成潜力，并提供了对支撑这些属性的分子机制的深入了解。