Objective

Marine actinomycetes from the genus Salinispora have an unexploited biotechnological potential. To accurately estimate their application potential however, data on their cultivation, including biomass growth kinetics, are needed but only incomplete information is currently available.

Results

This work provides some insight into the effect of temperature, salinity, nitrogen source, glucose concentration and oxygen supply on growth rate, biomass productivity and yield of Salinispora tropica CBN-440^T. The experiments were carried out in unbaffled shake flasks and agitated laboratory-scale bioreactors. The results show that the optimum growth temperature lies within the range 28–30 °C, salinity is close to sea water and the initial glucose concentration is around 10 g/L. Among tested nitrogen sources, yeast extract and soy peptone proved to be the most suitable. The change from unbaffled to baffled flasks increased the volumetric oxygen transfer coefficient (k_La) as did the use of agitated bioreactors. The highest specific growth rate (0.0986 h⁻¹) and biomass productivity (1.11 g/L/day) were obtained at k_La = 28.3 h⁻¹. A further increase in k_La was achieved by increasing stirrer speed, but this led to a deterioration in kinetic parameters.

Conclusions

Improvement of S. tropica biomass growth kinetics of was achieved mainly by identifying the most suitable nitrogen sources and optimizing k_La in baffled flasks and agitated bioreactors.

中文翻译：

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在摇瓶和机械搅拌生物反应器中实验室规模培养热带盐孢菌

客观的

盐生菌属的海洋放线菌具有未开发的生物技术潜力。然而，为了准确估计它们的应用潜力，需要关于它们的培养数据，包括生物质生长动力学，但目前只有不完整的信息可用。

结果

这项工作提供了一些关于温度、盐度、氮源、葡萄糖浓度和氧气供应对热带盐孢菌CBN-440 ^{T 的}生长速率、生物量生产力和产量的影响的一些见解。实验在无挡板的摇瓶和搅拌的实验室规模的生物反应器中进行。结果表明，最适生长温度在28~30 ℃，盐度接近海水，初始葡萄糖浓度在10 g/L左右。在测试的氮源中，酵母提取物和大豆蛋白胨被证明是最合适的。从无挡板烧瓶到有挡板烧瓶的变化增加了体积氧传递系数 (k _L a)，使用搅拌式生物反应器也是如此。最高比增长率（0.0986 h^-1 ) 和生物质生产力 (1.11 g/L/天) 在 k _L a = 28.3 h ^{-1 时获得}。通过增加搅拌器速度可以进一步增加 k _L a ，但这会导致动力学参数的恶化。

结论

主要通过确定最合适的氮源和优化挡板烧瓶和搅拌生物反应器中的k _L a 来改善S. tropica生物质生长动力学。