Spinal cord injury (SCI) causes patients paralysis and hard to recover. The therapeutic effects of current clinical drugs are accompanied by side effects. In recent years, stem cell therapy has attracted the attention of researchers. Human umbilical cord mesenchymal stem cells (hucMSCs) have been widely used in various diseases due to their excellent paracrine function. TNF-stimulated gene 6 (TSG-6), a secretion factor of stem cells, may play an important role in hucMSCs in the treatment of SCI. So we conducted an experiment to explore its effect. We first observed that the expression of TSG-6 increased in SCI rats after injected with hucMSCs. Then, we used siRNA to knowdown the expression of TSG-6. We treated SCI rats with TSG-6-knockdown hucMSCs. Without TSG-6 expression, hucMSCs treatment made the tissue recovery worse and the number of Nissl bodies less. Meanwhile, neutrophils infiltrated more in the damaged parts. Our research also proved that TSG-6 may help demyelination recovering and alleviate astrocytes gathering in the injury sites. Our study revealed that hucMSCs secreted TSG-6 may decrease the degeneration of myelin sheath, reduce inflammation, decrease neuron loss and promote tissue repair. These results provided a new therapeutic factor for the treatment of SCI.

脊髓损伤（SCI）导致患者麻痹并且难以康复。当前临床药物的治疗作用伴随有副作用。近年来，干细胞疗法引起了研究人员的关注。人脐带间充质干细胞（hucMSC）由于其优异的旁分泌功能而被广泛用于各种疾病。TNF刺激基因6（TSG-6）是干细胞的分泌因子，可能在hucMSC中治疗SCI中起重要作用。因此，我们进行了一项实验以探讨其效果。我们首先观察到在注射hucMSCs后SCI大鼠中TSG-6的表达增加。然后，我们使用siRNA来了解TSG-6的表达。我们用TSG-6基因敲低hucMSCs处理SCI大鼠。没有TSG-6表达，hucMSCs处理使组织恢复恶化，尼氏小体数目减少。同时，中性粒细胞更多地渗透到受损部位。我们的研究还证明，TSG-6可能有助于脱髓鞘的恢复并减轻聚集在损伤部位的星形胶质细胞。我们的研究表明hucMSC分泌的TSG-6可能减少髓鞘的变性，减少炎症，减少神经元丢失并促进组织修复。这些结果为SCI的治疗提供了新的治疗因素。减少神经元丢失并促进组织修复。这些结果为SCI的治疗提供了新的治疗因素。减少神经元丢失并促进组织修复。这些结果为SCI的治疗提供了新的治疗因素。