Abstract

Polyelectrolytes currently play an increasingly important role in antivirus therapy. Antiviral activity towards influenza virus, measles virus, herpes simplex virus type 1, and cytomegalovirus was demonstrated for the 6000 Da polyelectrolyte polyallylamine. A nontoxic polyallylamine concentration of 30 µM at which the compound retains its antiviral effect towards measles and influenza viruses but lacks any toxic effect on human cells was previously determined. It is well known, at the same time, that simultaneous virus exposure to physical environmental factors and chemical substances causes a more significant decrease in virus infectivity. Temperature is among these physical factors since thermal exposure causes virus inactivation. Analysis of virus thermal inactivation parameters is of high practical importance when it comes to the development of vaccines against influenza virus and to the study of how virus particles infectivity decreases on various surfaces. In this view, the study of kinetic and thermodynamical characteristics of influenza virus thermal inactivation in the presence of the antiviral preparation polyallylanime is of particular interest. The paper reports that thermal inactivation of influenza virus in the temperature range of 38–60°C in the presence of polyallylamine follows the first-order reaction kinetics. Thermodynamic parameters of influenza virus thermal inactivation evidence that influenza virus surface proteins are involved in the inactivation process as a result of their interaction with polyallylamine. The obtained results show that polyallylamine may be used to accelerate thermal inactivation of the influenza virus.

中文翻译：

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聚烯丙胺存在下 A/BANGKOK/1/1979(Н3N2) 病毒热灭活动力学

 抽象的

目前聚电解质在抗病毒治疗中发挥着越来越重要的作用。 6000 Da 聚电解质聚烯丙胺对流感病毒、麻疹病毒、1 型单纯疱疹病毒和巨细胞病毒具有抗病毒活性。先前已确定，无毒的聚烯丙胺浓度为 30 µM，在该浓度下，该化合物保留对麻疹和流感病毒的抗病毒作用，但对人体细胞没有任何毒性作用。同时众所周知，病毒同时暴露于物理环境因素和化学物质会导致病毒感染性更显着下降。温度是这些物理因素之一，因为热暴露会导致病毒失活。在开发流感病毒疫苗以及研究病毒颗粒在各种表面上的感染性如何降低时，病毒热灭活参数的分析具有非常重要的实际意义。由此看来，抗病毒制剂聚烯丙胺存在下流感病毒热灭活的动力学和热力学特征的研究特别令人感兴趣。该论文报道，在聚烯丙胺存在的情况下，流感病毒在 38-60°C 的温度范围内热灭活遵循一级反应动力学。流感病毒热灭活的热力学参数证明流感病毒表面蛋白由于与聚烯丙胺相互作用而参与灭活过程。所得结果表明聚烯丙胺可用于加速流感病毒的热灭活。