Ice-associated phototrophic taxa contribute significantly to Antarctic primary production and are crucial to ecosystem stability in the Southern Ocean. The quantity and quality of light required for photosynthesis is the single most influential driver of ice-associated algal communities. While the presence of ice and snow greatly reduces the irradiance reaching the ice-water interface, it is also the spectral quality that influences the phototrophy of ice-associated microalgae communities. Here we test the capability of three electrochemical microsensors to detect photosynthetically derived “stress” metabolites produced by the Antarctic diatom F. cylindrus. Following a period of dark incubation, this photo-physiological response differed with respect to the intensity and spectral quality of light during re-illumination. Exposure to blue light resulted in impairment in photosynthetic efficiency of PS_II (F_v/F_m) and resulted in the production of nitric oxide (NO), hydrogen peroxide (H₂O₂) and glucose exudation. A similar trend in metabolite production was observed when subjected to white light, but not during red or green illumination. These results indicate that rapid exposure to light and variation in spectral composition can cause significant stress that can be quantified using H₂O₂, NO and glucose microsensors. This metabolic overflow was triggered by the disruption of normal photosynthetic electron flow and it is proposed that the detection of extracellular metabolites can be directly attributed to intracellular activity.

与冰有关的光养类群对南极初级生产做出了重要贡献，对南大洋的生态系统稳定至关重要。光合作用所需的光的数量和质量是与冰相关的藻类群落的最有影响力的驱动力。尽管冰和雪的存在极大地降低了到达冰水界面的辐照度，但光谱质量也影响了与冰相关的微藻群落的光养作用。在这里，我们测试了三种电化学微传感器检测由南极硅藻F. cylindrus产生的光合作用衍生的“应激”代谢产物的能力。经过一段时间的黑暗孵育后，这种光生理反应在重新照明期间的光强度和光谱质量方面有所不同。暴露在蓝光下会损害PS _II（F _v / F _m）的光合作用效率，并导致产生一氧化氮（NO），过氧化氢（H ₂ O ₂）和葡萄糖渗出。当受到白光照射时，观察到类似的代谢产物生成趋势，但是在红色或绿色光照下则观察不到。这些结果表明，快速暴露于光和光谱成分的变化会引起显着的应力，可以使用H ₂ O ₂对其进行量化。，NO和葡萄糖微传感器。这种代谢的溢出是由正常的光合作用电子流的破坏触发的，并且提出细胞外代谢物的检测可以直接归因于细胞内活性。