Abstract

Microalgae have been studied for their potential of wastewater treatment as well as a promising source for biodiesel production. This study investigates the potential of microalgae to remove nutrients from domestic wastewater (DWW) while producing lipids-rich biomass for biodiesel production. Eight microalgae were cultivated in (DWW) to evaluate their nutrients removal capacity and biomass production. Total phosphorus (TP) of DWW reduced from 2 mg L⁻¹ to 0.02 mg L⁻¹ with the treatment efficiency of 99.15% and the highest performance was noted in Chlamydomonas reinhardtii (C. reinhardtii). For total nitrogen (TN), treatment efficiency climbed to 99.07%. It is reduced from 18.35 to 0.17 mg L⁻¹ recorded in C. reinhardtii and Chlorella pyrenoidosa (C. pyrenoidosa). On the other hand, all microalgae showed a high lipids-rich biomass in wastewater compared to BG11. The highest lipid content was 36.93% noted in Chlorella sorokiniana (C. sorokiniana). Fatty acids methyl ester (FAME) profiles showed a high content of palmitic C16:0, oleic C18:1 and stearic acids C18:0 in studied microalgae strains. In summary, microalgae envisage its potential application in integrated wastewater treatment and biodiesel production. In perspective, the authors focus on the validation of this bioprocess in pilot scale. Furthermore, the use of microalgae for other applications such CO₂ biosequestration and added value products.

Novelty statement: The present study investigates the potential of Moroccan microalgae as candidates to wastewater remediation and high biomass production with high lipid rate for biodiesel production.

摘要

已经研究了微藻的废水处理潜力以及生物柴油生产的有希望的来源。本研究调查了微藻从生活废水 (DWW) 中去除营养物质的潜力，同时为生物柴油生产生产富含脂质的生物质。在 (DWW) 中培养了八种微藻，以评估它们的养分去除能力和生物量生产。DWW的总磷（TP）从2 mg L ^-1降低到0.02 mg L ^-1，处理效率为99.15％，其中莱茵衣藻（C. reinhardtii）的处理效率最高。总氮（TN）处理效率攀升至99.07%。它从 18.35 减少到 0.17 mg L ^-1记录在C. reinhardtii和Chlorella pyrenoidosa (C. pyrenoidosa )。另一方面，与 BG11 相比，所有微藻在废水中都表现出高富含脂质的生物量。Chlorella sorokiniana (C. sorokiniana)中的脂质含量最高，为 36.93% 。脂肪酸甲酯 (FAME) 谱显示在研究的微藻菌株中棕榈酸 C16:0、油酸 C18:1 和硬脂酸 C18:0 含量较高。总之，微藻设想其在综合废水处理和生物柴油生产中的潜在应用。从长远来看，作者专注于在中试规模中验证这种生物过程。此外，将微藻用于其他应用，例如 CO ₂生物封存和附加值产品。

新颖性声明：本研究调查了摩洛哥微藻作为废水修复和高生物量生产的候选物以及高脂率用于生物柴油生产的潜力。