Background: Diosmin is a flavonoid obtained from the citrus fruits of the plants. Diosmin has blood lipid lowering activities, antioxidant activity, enhances venous tone and microcirculation, protects capillaries, mainly by reducing systemic oxidative stress.

Objective: The present study demonstrates the potential of Diosmin against the enzymes aldose reductase, α-glucosidase, and α-amylase involved in diabetes and its complications by in vitro evaluation and reverse molecular docking studies.

Methods: The assay of aldose reductase was performed by using NADPH as starting material and DL-Glyceraldehyde as a substrate. DNS method was used for alpha amylase inhibition and in alpha glucosidase inhibitory activity p-nitrophenyl glucopyranoside (pNPG) was used as substrate. The reverse molecular docking studies was performed by using Molegro software (MVD) with grid resolution of 30 Å.

Results: Diosmin shows potent inhibitory effect against aldose reductase (IC50:333.88±0.04 μg/mL), α-glucosidase (IC50:410.3±0.01 μg/mL) and α-amylase (IC50: 404.22±0.02 μg/mL) respectively. The standard drugs shows moderate inhibitory activity for enzymes. The MolDock Score of Diosmin was -224.127 against aldose reductase, -168.17 against α-glucosidase and - 176.013 against α-amylase respectively, which was much higher than standard drugs.

Conclusion: From the result it was concluded that diosmin was a potentially inhibitor of aldose reductase, alpha amylase and alpha glucosidase enzymes then the standard drugs and it will be helpful in the management of diabetes and its complications. This will also be benevolent to decrease the socio economical burden on the middle class family of the society.

背景：地奥司明是一种从植物的柑橘类果实中提取的黄酮类化合物。地奥司明具有降血脂、抗氧化活性，增强静脉张力和微循环，保护毛细血管，主要通过减少全身氧化应激。

目的：本研究通过体外评估和反向分子对接研究证明了地奥司明对参与糖尿病及其并发症的醛糖还原酶、α-葡萄糖苷酶和α-淀粉酶的潜力。

方法：以NADPH为起始原料，DL-甘油醛为底物，进行醛糖还原酶的测定。DNS 方法用于抑制α 淀粉酶，在α 葡萄糖苷酶抑制活性中，对硝基苯基吡喃葡萄糖苷(pNPG) 用作底物。通过使用网格分辨率为 30 Å 的 Molegro 软件 (MVD) 进行反向分子对接研究。

结果：地奥司明对醛糖还原酶（IC50：333.88±0.04 μg/mL）、α-葡萄糖苷酶（IC50：410.3±0.01 μg/mL）和α-淀粉酶（IC50：分别为404.22±0.02 μg/mL）显示出强效抑制作用。标准药物对酶具有中等抑制活性。Diosmin 的 MolDock 评分对醛糖还原酶为 -224.127，对 α-葡萄糖苷酶为 -168.17，对 α-淀粉酶为 -176.013，远高于标准药物。

结论：结果表明地奥司明是醛糖还原酶、α-淀粉酶和α-葡萄糖苷酶的潜在抑制剂，是标准药物，有助于糖尿病及其并发症的治疗。这也有利于减轻社会中产阶级家庭的社会经济负担。