ABSTRACT

An ozonation study was conducted for inactivating Fusarium oxysporum, which causes Fusarium wilt, the most serious soil-borne disease in hydroponic cultivation systems. Samples of conidial suspensions of F. oxysporum were treated with ozone gas generated at two ozone gas-phase compositions: oxygen feed and air feed. Oxygen feed ozonation resulted in 0% viability of the pathogen for all ozone dosages tested, including the lowest measurable ozone dosage of 1 mgO₃/L. For air feed ozonation, multiple regression with the Akaike information criterion (AIC) for model selection was used to find the effects of the ozone dosage ${\mathrm{O}}_3$ (mgO₃/L) and the duration of ozonation (time), ${\mathrm{O}}_3$ (min), on the viability (%) of the pathogen at 5 °C and 21 °C. At 5 °C, the viability was found to be linear in ${\mathrm{O}}_3$; at 21 °C, the viability was linear in ${\mathrm{O}}_3$ and quadratic in ${\mathrm{O}}_3$, and the interaction of ${\mathrm{O}}_3$ and ${\mathrm{O}}_3$ was also found to be significant.

The novelty of this study was to use oxygen as feed gas for ozonation for disinfestation of Fusarium oxysporum. This is a significantly different approach from any previously published work on fungal plant pathogen disinfestation. Ozonation with air feed was effective only at high ozone dosages and low temperature (5 °C). The findings of this research clearly show and imply that high-concentration ozone, carried by oxygen feed, is an efficient and sustainable alternative to chemical fungicides in the treatment of Fusarium wilt in hydroponic nutrient solutions because ozone decomposes quickly to oxygen, resulting in an environmentally safe and nontoxic residue.

摘要

进行了一项臭氧化研究以灭活尖孢镰刀菌，它会导致枯萎病，这是水培系统中最严重的土传病害。尖孢镰刀菌的分生孢子悬浮液样品用在两种臭氧气相组成下产生的臭氧气体处理：氧气进料和空气进料。对于所有测试的臭氧剂量，包括最低可测量臭氧剂量 1 mgO ₃ /L ，氧气进料臭氧化导致病原体的存活率为 0% 。对于空气进料臭氧化，使用 Akaike 信息准则 (AIC) 进行模型选择的多元回归来找出臭氧剂量的影响${\mathrm{○}}_3$(mgO ₃ /L)和臭氧化持续时间(时间)，${\mathrm{○}}_3$(min)，关于病原体在 5°C 和 21°C 下的存活率 (%)。在 5 °C 时，发现活力呈线性${\mathrm{○}}_3$; 在 21 °C 时，活力呈线性${\mathrm{○}}_3$ 和二次 ${\mathrm{○}}_3$, 和相互作用 ${\mathrm{○}}_3$ 和 ${\mathrm{○}}_3$ 也被发现意义重大。

这项研究的新颖之处在于使用氧气作为臭氧化的原料气对尖孢镰刀菌进行杀虫。这是一种与之前发表的任何关于真菌植物病原体灭虫的工作显着不同的方法。空气进料的臭氧化仅在高臭氧剂量和低温（5°C）下有效。这项研究的结果清楚地表明并暗示，在水培营养液中处理枯萎病时，由氧气进料携带的高浓度臭氧是一种有效且可持续的化学杀菌剂替代品，因为臭氧会迅速分解成氧气，从而对环境产生不利影响。安全无毒残留物。