Abstract

Origanum vulgare L. is an important aromatic medicinal plant, which has many medicinal properties and is used in the cosmetics and pharmaceutical industries. This study aims to evaluate the chemical composition and anti-microbial activities of essential oil extracted from aerial parts of a wild-growing O. vulgare L. The plant has been collected from the North-Western Himalayas region (Himachal Pradesh, India) and essential oil from its aerial part has been isolated through hydro-distillation. Gas chromatography/mass spectroscopy (GC/MS) analysis of the essential oil has been carried out to reveal its chemical composition. A total of twenty-four constituents have been identified, out of which eight constituents were different than reported in other studies, most probably due to geographical (height, temperature, soil, flora, and fauna) and geological (plants and animals) conditions. This essential oil has been dominated by oxygenated sesquiterpenes-38.08 %, followed by phenolic monoterpenoids-20.39 %, sesquiterpene hydrocarbon-14.4 %, monoterpene hydrocarbon-5.75 %, aliphatic alcohols-4.35 %, oxygenated monoterpene-3.99 %, polycyclic aromatic hydrocarbon-2.55 %, and sesquiterpenoid alcohol-2.26 %. Caryophyllene oxide (38.08 %) and thymol (20.39 %) have been found as major chemical components of essential oil. Anti-microbial activity of essential oil of O. vulgare has shown the highest zone of inhibition against the fungus Aspergillus niger (14 mm) and lowest against the Gram +ve bacteria Staphylococcus aureus (6 mm) at 600 ppm concentration among the all tested strains.

摘要

牛至属植物是重要的芳香药用植物，其具有许多药用特性，并用于化妆品和制药工业。这项研究旨在评估从野生生长的普通麦的空中部分提取的精油的化学成分和抗菌活性。L.该植物是从西北喜马拉雅山脉地区（印度，希马克马，印度）收集的，其空中部分的精油已通过加氢蒸馏分离出来。已经对精油进行了气相色谱/质谱（GC / MS）分析，以揭示其化学成分。总共确定了二十四种成分，其中八种成分与其他研究报告不同，这很可能是由于地理条件（高度，温度，土壤，动植物）和地质条件（动植物）所致。该精油的主要成分是氧化倍半萜烯-38.08％，其次是酚类单萜20.39％，倍半萜烃-14.4％，单萜烃-5.75％，脂族醇-4.35％，氧化单萜-3.99％，多环芳烃-2 。55％，倍半萜醇为2.26％。已发现石竹烯氧化物（38.08％）和百里香酚（20.39％）是精油的主要化学成分。香精油的抗菌活性O.大麦已经显示出抑制针对真菌的最高区黑曲霉（14毫米），最低对抗革兰氏+细菌已经金黄色葡萄球菌的所有测试的菌株中在600ppm的浓度（6毫米）。