Abstract

The herb, Elettariopsis triloba (Gagnep.) Loes. (syn. Amomum trilobum Gagnep. or Elettariopsis trilobum (Gagnep.), has been used ethnomedically for the treatment of rabies, inflammation and amelioration of microbial infections. We report herein the chemical constituents and antimicrobial activity of the rhizome essential oil of E. triloba from north-central Vietnam. The technique of gas chromatography (GC) and gas chromatography coupled with mass spectrometry (GC/MS) was used to analyze the oil sample while the microdilution assay was employed to determine the antimicrobial efficacy. Monoterpene compounds including camphene (23.2 %), fenchyl acetate (12.7 %), bornyl acetate (10.6 %), α-pinene (5.7 %) and limonene (5.1 %) were the main constituents of the rhizome essential oil. The essential oil displayed antimicrobial activity only towards Gram-positive bacteria of Enterococcus faecalis ATCC 299212, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 14579 with minimum inhibitory concentration (MIC) values of 16.0 μg/mL, 256 μg/mL and 32.0 μg/mL, respectively. The oil also inhibited the growth of the yeast, Candida albicans ATCC 10231, with a MIC value of 16.0 μg/mL. The chemical constituents and antimicrobial activity of essential oil from E. triolba were being reported for the first time and validation of the ethnomedicinal usage of the plant.

摘要

草本，Elettariopsis triloba（Gagnep。）Loes。（SYN。砂仁trilobum Gagnep。或Elettariopsis trilobum（Gagnep。），已经ethnomedically用于狂犬病，炎症和微生物感染的改善的治疗。我们报告本文中的化学成分及根茎精油的抗菌活性E.榆叶梅来自越南中北部。气相色谱（GC）和气相色谱-质谱联用技术（GC / MS）用于分析油样，而微量稀释法用于确定抗菌效果。根茎精油的主要成分包括单萜类化合物，其中包括樟脑（23.2％），醋酸芬基酯（12.7％），醋酸冰片酯（10.6％），α-pine烯（5.7％）和li烯（5.1％）。精油仅对粪肠球菌ATCC 299212，金黄色葡萄球菌ATCC 25923和蜡状芽孢杆菌的革兰氏阳性细菌显示出抗菌活性ATCC 14579的最小抑菌浓度（MIC）值分别为16.0μg/ mL，256μg/ mL和32.0μg/ mL。该油还抑制了酵母（白色念珠菌ATCC 10231）的生长，MIC值为16.0μg/ mL。首次报道了来自三叶草的香精油的化学成分和抗菌活性，并验证了该植物的人种学用途。