Abstract

The present study aims to determine the chemical composition of Pistaca atlantica Desf. collected in the Khenifra region (Morocco). Compounds chemicals of the essential oil were analyzed by gas chromatography/mass spectrometry (GC/MS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing assay power (FRAP) methods were used to evaluate antioxidant activity. Antibacterial activity of the essential oil was tested Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii and Serratia marcescens, Gram-positive bacteria, Staphylococcus epidermidis and Staphylococcus aureus. The yield of essential oil was 0.32 ± 0.01 % (w/w) and its analysis by GC/MS has identified 29 components with the dominance of terpinen-4-ol (26.20 %) followed by spathulenol (7.73 %), α-cadinol (6.91 %), epi- α-muurolol (5.61 %), o-cymene (5.33 %), caryophyllene oxide (4.37 %), α-terpineol (4.32 %) and α-pinene (3.43 %). The result of the DPPH and FRAP assay gives IC₅₀ and EC₅₀ values of 82.88 and 1.71 mg/mL respectively. The oxygenated monoterpene and sesquiterpene showed the highest antibacterial activity. The P. atlantica essential oil can be used as a preservative in the food industry to increase food safety and reduce food pathogens risks.

摘要

本研究旨在确定开心果的化学成分。收集在Khenifra地区（摩洛哥）。通过气相色谱/质谱法（GC / MS）分析香精油的化合物化学物质。2,2-二苯基-1-吡啶并肼基（DPPH）和还原铁含量测定方法（FRAP）用于评估抗氧化活性。测试了香精油的抗菌活性革兰氏阴性菌大肠杆菌，肺炎克雷伯菌，鲍曼不动杆菌和粘质沙雷氏菌，革兰氏阳性菌，表皮葡萄球菌和金黄色葡萄球菌。精油的产率为0.32±0.01％（w / w），通过GC / MS进行分析后，鉴定出29种成分，其中以萜品烯-4-醇（26.20％）为主要成分，其次是芦丁烯醇（7.73％），α-cadinol （6.91％），表-α-莫洛洛尔（5.61％），邻-异丙基苯（5.33％），石竹烯氧化物（4.37％），α-松油醇（4.32％）和α-pine烯（3.43％）。DPPH和FRAP分析的结果分别得出IC ₅₀和EC ₅₀值分别为82.88和1.71 mg / mL。氧化的单萜和倍半萜显示出最高的抗菌活性。该P.王者精油可以作为防腐剂在食品工业中，以增加食品安全和减少食品病原体的风险。