Evaluation of virulence potential of Aspergillus tubingensis and subsequent biochemical and enzymatic defense response of cotton,Microscopy Research and Technique

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Evaluation of virulence potential of Aspergillus tubingensis and subsequent biochemical and enzymatic defense response of cotton
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-05-17 , DOI: 10.1002/jemt.23832
Maria Khizar ₁ , Urooj Haroon ₁ , Asif Kamal ₁ , Wardah Inam ₁ , Hassan Javed Chaudhary ₁ , Muhammad Farooq Hussain Munis ₁

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Aspergillus tubingensis is a causative known pathogen of various important crops, worldwide. The existing study was aimed to examine the virulence potential of A. tubingensis on resistant (NIA-Sadori) and susceptible (CIM-573) cultivars of cotton. For this purpose, both cultivars were inoculated with pathogen and altered morphology of diseased leaves was observed with light and scanning electron microscope. Disease severity was measured and estimated to be 68.7 and 27.1% in susceptible and resistant cultivars, respectively. To understand and compare defense mechanism of resistant and susceptible cultivars, different biochemical and enzymatic changes were observed. After the infection of A. tubingensis, increase in the concentrations of sugar, total protein, proline, phenol, and phenylalanine ammonia lyase (PAL) was more prominent in resistant cultivar, than the susceptible one. Moreover, due to increased number of dead cells, significantly higher electrolyte leakage was detected in susceptible cultivar. Principal component analysis confirmed the effect of A. tubingensis on growth attributes and various physiological and biochemical activities of cotton. These findings help us to suggest a possible role of proline content, protein content, and PAL activity in resistance mechanism of Cotton plant.

中文翻译：

塔宾曲霉毒力评价及棉花后续生化和酶促防御反应

塔宾曲霉是世界范围内各种重要作物的已知病原体。现有研究旨在检查塔宾曲霉对抗性（NIA-Sadori）和易感性（CIM-573）棉花品种的毒力潜力。为此，两个栽培品种都接种了病原体，并用光学和扫描电子显微镜观察了病叶形态的改变。在易感和抗性品种中，测量并估计疾病严重程度分别为 68.7% 和 27.1%。为了了解和比较抗性和易感品种的防御机制，观察了不同的生化和酶学变化。塔宾曲霉感染后, 糖、总蛋白、脯氨酸、苯酚和苯丙氨酸解氨酶 (PAL) 浓度的增加在抗性品种中比敏感品种更显着。此外，由于死细胞数量增加，在易感品种中检测到显着更高的电解质泄漏。主成分分析证实了塔宾曲霉对棉花生长特性和各种生理生化活性的影响。这些发现有助于我们提出脯氨酸含量、蛋白质含量和 PAL 活性在棉花植物抗性机制中的可能作用。

更新日期：2021-05-17

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