ABSTRACT

Background

Lipid-polymer hybrid nanoparticles (LPHNP) are used for drug delivery due to their high advantages over polymeric or lipid nanoparticles.

Materials and methods

Tamoxifen-Curcumin-loaded-LPHNPs (Tmx-Cu-LPHNPs) were synthetized by the ionic gelation method, and after characterization and calculation of the encapsulation efficiency, its antioxidant power, anti-cancer and anti-metastatic effects were examined.

Results

Tmx-Cu-LPHNPswith size of 232.12 nm, PDI: 0.22, ζ-p: 35.12 mV and encapsulation efficiencies92% and 74% for Cu and Tmx (respectively) inhibited cancer cells more strongly than normal cells.In addition, Tmx-Cu-LPHNPsinhibited ABTS (IC50: 998.125 μg/ml), DPPH (IC50:164.87 μg/ml) free radicals as well as reducing iron ions.Up regulation of P53 and Caspase-9, flow cytometry and AO/PI staining demonstrated proapoptotic effects of Tmx-Cu-LPHNPs. Suppression of metastasis in cancer cells confirmed by scratch assay and decreased expression of metalloproteinase (MMP-2, 9) genes.

Conclusion

Tmx-Cu-LPHNPs can be a promising factor in the treatment of cancer with appropriate therapeutic effects.

中文翻译：

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姜黄素和他莫昔芬共包裹在脂质-壳聚糖杂化纳米颗粒中用于癌症治疗

摘要

背景

脂质-聚合物混合纳米粒子 (LPHNP) 因其优于聚合物或脂质纳米粒子的高度优势而被用于药物递送。

材料和方法

采用离子凝胶法合成了三苯氧胺-姜黄素负载-LPHNPs（Tmx-Cu-LPHNPs），对其包封率进行表征和计算后，检测其抗氧化能力、抗癌和抗转移作用。

结果

Tmx-Cu-LPHNPs 尺寸为 232.12 nm，PDI：0.22，ζ-p：35.12 mV，Cu 和 Tmx 的封装效率分别为 92% 和 74%，比正常细胞更强烈地抑制癌细胞。此外，Tmx-Cu- LPHNPs 抑制 ABTS (IC50: 998.125 μg/ml)、DPPH (IC50:164.87 μg/ml) 自由基以及还原铁离子。P53 和 Caspase-9 的上调、流式细胞术和 AO/PI 染色证明了 Tmx 的促凋亡作用-Cu-LPHNP。划痕试验证实了癌细胞转移的抑制和金属蛋白酶 (MMP-2, 9) 基因的表达降低。

结论

Tmx-Cu-LPHNPs 可以成为治疗癌症的有希望的因素，具有适当的治疗效果。