Evaluation of saliva as a source of accurate whole-genome and microbiome sequencing data,Genetic Epidemiology

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Evaluation of saliva as a source of accurate whole-genome and microbiome sequencing data
Genetic Epidemiology ( IF 1.7 ) Pub Date : 2021-05-16 , DOI: 10.1002/gepi.22386
Anthony F Herzig ₁ , Lourdes Velo-Suárez _{1,

2} , Gaëlle Le Folgoc ₁ , Anne Boland _{3,

4} , Hélène Blanché _{4,

5} , Robert Olaso _{3,

4} , Liana Le Roux ₆ , Christelle Delmas ₇ , Marcel Goldberg ₈ , Marie Zins ₈ , Franck Lethimonnier ₉ , Jean-François Deleuze _{3,

4,

5,

10} , Emmanuelle Génin _{1,

11}

Affiliation

Univ Brest, EFS, UMR 1078, GGB, Inserm, Brest, France.
Brest Center for Microbiota Analysis (CBAM), CHU Brest, Brest, France.
National Center for Research in Human Genomics (CNRGH), François Jacob Institute of Biology, CEA, Paris-Saclay University, Evry, France.
Laboratory of Excellence GENMED (Medical Genomics), Paris, France.
Fondation Jean Dausset-CEPH, Paris, France.
Clinical Investigation Center 1412, Inserm, CHU Brest, Brest, France.
Clinical Research Center, INSERM, Paris, France.
Inserm-Paris Saclay University, University of Paris, Villejuif, France.
National Alliance for Life and Health Sciences (Aviesan), Multiorganism thematic institute, Health technologies, INSERM, Paris, France.
Center of Reference, Innovation and Expertize (CREFIX), US39, French Atomic Energy and Alternative Energies Commission, Evry, France.
CHU Brest, Brest, France.

This study sets out to establish the suitability of saliva-based whole-genome sequencing (WGS) through a comparison against blood-based WGS. To fully appraise the observed differences, we developed a novel technique of pseudo-replication. We also investigated the potential of characterizing individual salivary microbiomes from non-human DNA fragments found in saliva.

中文翻译：

评估唾液作为准确全基因组和微生物组测序数据的来源

本研究旨在通过与基于血液的全基因组测序 (WGS) 进行比较来确定基于唾液的全基因组测序 (WGS) 的适用性。为了充分评估观察到的差异，我们开发了一种新的伪复制技术。我们还研究了从唾液中发现的非人类 DNA 片段表征个体唾液微生物组的潜力。

更新日期：2021-06-23

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