Leptospirosis disease was caused by rat urine which contains the genus Leptospira bacteria. In this study, the fabrication of Pd-Fe-doped polyaniline nanocomposite thin films for the determination of the genus Leptospira bacteria thin films has been investigated. Pd-Fe-doped polyaniline nanocomposite thin films were fabricated by sol–gel spin coating method. The electrode sensors were immersed in the Leptospira solution. The resulting materials were investigated using field-emission scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and current–voltage measurement. The atomic force microscopy images show the specific morphology films’ structure for Leptospira detection, whereas the field-emission scanning electron microscopy image shows the irregularity of clump nanoparticles in thin film surfaces. Transmission electron microscopy result shows that metal alloy (Fe-Pd) embedded in the polymer matrix. Current–voltage measurement with and without incubation of the thin film into Leptospira solution was done to show the relationship between concentration bacteria versus current. The result shows that polyaniline-Fe_0.4-Pd_0.6 nanocomposite thin film has higher sensitivity in detecting Leptospira, where it has performed with the highest percentage of the sensitivity of 16.9%. Besides that, selectivity tests were conducted to distinguish the existence of Leptospira, Pseudomonas aeruginosa, and Staphylococcus aureus bacteria. These results confirm the potentials of polyaniline metal alloys’ nanocomposite thin films to be used for Leptospira bacteria detection in water.

钩端螺旋体病是由含有钩端螺旋体属细菌的大鼠尿液引起的。在这项研究中，已研究了用于确定钩端螺旋体细菌薄膜的Pd-Fe掺杂聚苯胺纳米复合薄膜的制备。溶胶-凝胶旋涂法制备了Pd-Fe掺杂的聚苯胺纳米复合薄膜。将电极传感器浸入钩端螺旋体溶液中。使用场发射扫描电子显微镜，原子力显微镜，透射电子显微镜和电流-电压测量对所得材料进行了研究。原子力显微镜图像显示钩端螺旋体的特定形态膜结构检测，而场发射扫描电子显微镜图像显示了薄膜表面团块纳米颗粒的不规则性。透射电子显微镜结果表明，金属合金（Fe-Pd）嵌入聚合物基体中。在有或没有将薄膜孵育到钩端螺旋体溶液中的情况下进行电流-电压测量，以显示细菌浓度与电流之间的关系。结果表明，聚苯胺-Fe _0.4 -Pd _0.6纳米复合薄膜在检测钩端螺旋体中具有较高的灵敏度，在该领域中，其灵敏度最高，为16.9％。除此之外，还进行了选择性测试以区分钩端螺旋体的存在，铜绿假单胞菌和金黄色葡萄球菌细菌。这些结果证实了聚苯胺金属合金的纳米复合薄膜在水中钩端螺旋体细菌检测中的潜力。