Drug development from medicinal plants by using various hydroalcoholic solvents has been an emerging trend in the last few decades. Utilisation of plant stem for this purpose is a one-time use and causes destruction of natural resources. Hence, it is necessary to optimize the hydroalcoholic composition for obtaining the best extraction as a pre-work of any drug development. Tinospora cordifolia stem which is a rich source of alkaloids is considered for the present study. The aim of the study was to find out the best suitable hydroalcoholic composition for the maximum extraction of the therapeutically active alkaloid berberine. For this purpose, hydroalcoholic solvents of different ratios were used and extracts obtained thereafter were examined by high-performance thin-layer chromatography (HPTLC) for the quantification of berberine in those extracts. A simple, precise and robust HPTLC method was developed and validated for the quantification of berberine in different hydroalcoholic extracts. The study revealed that 60:40 (V/V) alcohol–water compositions were the best suited for berberine extraction with the highest yield of 0.642%. On the basis of linear calibration curve for berberine in the range of 30–100 ng, an average recoveries of berberine for different hydroalcoholic extracts were in the range between 100.66 and 104%. From the quantification result, it may be concluded that alcohol–water (60:40, V/V) may be considered for the development of drugs based on berberine.

在过去的几十年中，通过使用各种水醇溶剂从药用植物开发药物已成为一种新兴趋势。为此目的，一次性使用植物茎会导致自然资源的破坏。因此，有必要优化水醇组合物以获得最佳提取，这是任何药物开发的前期工作。田鼠本研究考虑了丰富的生物碱来源的茎。该研究的目的是找到最适合的水醇组合物，以最大程度地提取治疗活性生物碱小ber碱。为此目的，使用不同比例的水醇溶剂，然后通过高效薄层色谱法（HPTLC）检查获得的提取物，以定量那些提取物中的小ber碱。开发了一种简单，精确和可靠的HPTLC方法，并验证了该方法用于定量不同水醇提取物中的小ber碱的含量。研究表明60:40（V / V）酒精-水组合物最适合黄连素的提取，最高收率为0.642％。根据小ber碱在30–100 ng范围内的线性校准曲线，不同水醇提取物的小ber碱平均回收率在100.66％至104％之间。从定量结果可以得出结论，可以考虑使用酒精水（60：40，V / V）来开发基于小ber碱的药物。