Background

The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance.

Objective

The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry.

Results

We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin.

Conclusion

We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR.

中文翻译：

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姜黄素通过调节ROS /NF-κB信号通路抑制氧化应激，从而保护糖尿病性视网膜病变的视网膜血管内皮细胞

背景

即使在糖尿病性视网膜病（DR）的早期，视网膜血管内皮细胞也可能受到氧化应激的损害。本研究旨在探讨姜黄素在高糖环境下对大鼠视网膜血管内皮细胞（RRVECs）的保护作用。

客观的

通过vWF和CD31免疫荧光表达鉴定并表征了培养的RRVEC。通过电泳迁移率分析（EMSA），免疫组化和Western blot检测ROS /NF-κB信号通路的激活。流式细胞仪检测RRVECs的凋亡。

结果

我们发现姜黄素通过流式细胞仪减少了暴露于高葡萄糖的RRVECs中的活性氧（ROS）并减轻了其凋亡。结果表明，姜黄素能明显抑制高糖浓度引起的RRVECs中NF-κB和磷酸化NF-κB活性的增加。

结论

我们得出的结论是姜黄素可以通过调节NF-κB信号来抑制氧化应激，从而保护DR中的RRVECs。