This study aimed to identify the physiological responses of house cricket females following short-term exposure to relatively low dietary doses of graphene oxide (GO, 20 μg · g⁻¹ food), silver (Ag, 400 μg · g⁻¹ food) nanoparticles (NPs), or graphene oxide‑silver nanoparticle composite (GO-AgNPs, 20: 400 μg · g⁻¹ food). Energy intake and distribution were measured on the third, sixth, and tenth day. A semi-quantitative API®ZYM assay of digestive enzyme fingerprints was performed on the third and tenth day of continuous treatment. Physicochemical properties of the NPs were obtained by combining SEM, EDX spectrometry, AFM, and DLS techniques.

The obtained results showed decreased energy consumption, particularly assimilation as an early response to dietary NPs followed by compensatory changes in feeding activity leading to the same consumption and assimilation throughout the experimental period (10 days). The increased activities of digestive enzymes in NP-treated females compared to the control on the third day of the experiment suggest the onset of compensatory reactions of the day. Moreover, the insects treated with GO-AgNP composite retained more body water, suggesting increased uptake. The observed changes in the measured physiological parameters after exposure to NPs are discussed in light of hormesis.

这项研究旨在确定家女性在短期暴露于相对较低饮食剂量的氧化石墨烯（GO，20μg·g ^-1食物），银（Ag，400μg·g ^-1食物）纳米颗粒后的生理反应。（NPs）或氧化石墨烯-银纳米颗粒复合材料（GO-AgNPs，20：400μg·g ^-1食物）。在第三天，第六天和第十天测量能量的摄入和分配。在连续治疗的第三天和第十天对消化酶指纹图谱进行半定量API®ZYM分析。NP的理化性质是通过结合SEM，EDX光谱，AFM和DLS技术获得的。

获得的结果表明，能量消耗降低，特别是对日粮NP的早期反应产生的同化作用，随后是摄食活动的补偿性变化，导致整个实验期间（10天）的消耗和同化作用相同。在实验的第三天，与对照组相比，NP处理的雌性中消化酶的活性增加，表明这一天发生了代偿性反应。此外，用GO-AgNP复合物处理的昆虫保留了更多的体内水分，表明摄取量增加了。讨论了暴露于NP后测得的生理参数变化。