Cauliflower mosaic virus protein P6-TAV plays a major role in alteration of aphid vector feeding behaviour but not performance on infected Arabidopsis,Molecular Plant Pathology

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Cauliflower mosaic virus protein P6-TAV plays a major role in alteration of aphid vector feeding behaviour but not performance on infected Arabidopsis
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2021-05-15 , DOI: 10.1111/mpp.13069
Quentin Chesnais ₁ , Maxime Verdier ₁ , Myriam Burckbuchler ₁ , Véronique Brault ₁ , Mikhail Pooggin ₂ , Martin Drucker ₁

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Emerging evidence suggests that viral infection modifies host plant traits that in turn alter behaviour and performance of vectors colonizing the plants in a way conducive for transmission of both nonpersistent and persistent viruses. Similar evidence for semipersistent viruses like cauliflower mosaic virus (CaMV) is scarce. Here we compared the effects of Arabidopsis infection with mild (CM) and severe (JI) CaMV isolates on the feeding behaviour (recorded by the electrical penetration graph technique) and fecundity of the aphid vector Myzus persicae. Compared to mock-inoculated plants, feeding behaviour was altered similarly on CM- and JI-infected plants, but only aphids on JI-infected plants had reduced fecundity. To evaluate the role of the multifunctional CaMV protein P6-TAV, aphid feeding behaviour and fecundity were tested on transgenic Arabidopsis plants expressing wild-type (wt) and mutant versions of P6-TAV. In contrast to viral infection, aphid fecundity was unchanged on all transgenic lines, suggesting that other viral factors compromise fecundity. Aphid feeding behaviour was modified on wt P6-CM-, but not on wt P6-JI-expressing plants. Analysis of plants expressing P6 mutants identified N-terminal P6 domains contributing to modification of feeding behaviour. Taken together, we show that CaMV infection can modify both aphid fecundity and feeding behaviour and that P6 is only involved in the latter.

中文翻译：

花椰菜花叶病毒蛋白 P6-TAV 在改变蚜虫的摄食行为中起主要作用，但对受感染的拟南芥没有影响

新出现的证据表明，病毒感染会改变宿主植物的特性，进而改变以有利于非持久性和持久性病毒传播的方式在植物中定殖的载体的行为和性能。花椰菜花叶病毒 (CaMV) 等半持久性病毒的类似证据很少。在这里，我们比较了拟南芥感染与轻度 (CM) 和重度 (JI) CaMV 分离株对蚜虫载体桃蚜的摄食行为（通过电穿透图技术记录）和繁殖力的影响. 与模拟接种的植物相比，CM 和 JI 感染的植物的摄食行为发生了类似的变化，但只有 JI 感染的植物上的蚜虫的繁殖力降低。为了评估多功能 CaMV 蛋白 P6-TAV 的作用，在转基因拟南芥上测试了蚜虫的摄食行为和繁殖力表达野生型 (wt) 和突变型 P6-TAV 的植物。与病毒感染相比，所有转基因品系的蚜虫繁殖力没有变化，这表明其他病毒因素会影响繁殖力。蚜虫的摄食行为在 wt P6-CM- 上被改变，但在 wt P6-JI 表达植物上没有改变。对表达 P6 突变体的植物的分析确定了有助于改变摄食行为的 N 末端 P6 结构域。总之，我们表明 CaMV 感染可以改变蚜虫的繁殖力和摄食行为，并且 P6 仅参与后者。

更新日期：2021-07-22

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