当前位置：
X-MOL 学术
›
Microbiologyopen
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The effect of calcium palmitate on bacteria associated with infant gut microbiota
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-05-15 , DOI: 10.1002/mbo3.1187 Lu Wang 1 , Gabriela Bravo-Ruiseco 1 , Randolph Happe 2 , Tao He 2 , Jan Maarten van Dijl 1 , Hermie J M Harmsen 1
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-05-15 , DOI: 10.1002/mbo3.1187 Lu Wang 1 , Gabriela Bravo-Ruiseco 1 , Randolph Happe 2 , Tao He 2 , Jan Maarten van Dijl 1 , Hermie J M Harmsen 1
Affiliation
|
Gut microbiota development in formula‐fed and breast‐fed infants is known to differ. This could relate to the usage of unmodified vegetable oil instead of mammalian fat in infant formula (IF), causing the enhanced formation of the poorly soluble soap calcium palmitate (CP) in the infant's gut. Here we investigate in vitro the possible influence of CP on the infant gut bacteria. The growth of several bacterial species dominant in the infant's gut was analyzed by culturing in media with CP. Faecalibacterium prausnitzii as a sensitive representative was analyzed in detail by scanning transmission electron microscopy, membrane staining, gas chromatography, and microbial fuel cell experiments. Of all bacteria tested, the growth of several bifidobacteria and F. prausnitzii was reduced at 0.01 mg/ml CP, Bifidobacterium infantis stopped growing completely. CP reduced the cell envelope thickness of F. prausnitzii, disturbed the cell membrane fatty acids and function of membrane proteins involved in electron transport. CP inhibited the growth of bifidobacteria and faecalibacteria. This suggests that modification of fat in IF may benefit the development of the gut microbiota in formula‐fed infants by supporting the colonization of important beneficial bacteria in early life. Future clinical studies are needed to confirm this.
中文翻译：
棕榈酸钙对与婴儿肠道微生物群相关的细菌的影响
众所周知，配方奶喂养和母乳喂养婴儿的肠道微生物群发育存在差异。这可能与在婴儿配方奶粉 (IF) 中使用未改性植物油代替哺乳动物脂肪有关，导致婴儿肠道中难溶性棕榈酸钙 (CP) 的形成增加。在这里，我们在体外研究 CP 对婴儿肠道细菌的可能影响。通过在含有 CP 的培养基中培养来分析在婴儿肠道中占优势的几种细菌种类的生长。通过扫描透射电镜、膜染色、气相色谱和微生物燃料电池实验对作为敏感代表的普拉氏粪杆菌进行了详细分析。在所有测试的细菌中，几种双歧杆菌和F. prausnitzii的生长在 0.01 mg/ml CP 时减少，婴儿双歧杆菌完全停止生长。CP降低了F. prausnitzii的细胞包膜厚度，扰乱了细胞膜脂肪酸和参与电子传递的膜蛋白的功能。CP抑制双歧杆菌和粪杆菌的生长。这表明 IF 中脂肪的改变可能通过支持重要有益细菌在生命早期的定植，从而有利于配方奶喂养婴儿肠道微生物群的发育。需要未来的临床研究来证实这一点。
更新日期：2021-05-17
中文翻译：
棕榈酸钙对与婴儿肠道微生物群相关的细菌的影响
众所周知，配方奶喂养和母乳喂养婴儿的肠道微生物群发育存在差异。这可能与在婴儿配方奶粉 (IF) 中使用未改性植物油代替哺乳动物脂肪有关，导致婴儿肠道中难溶性棕榈酸钙 (CP) 的形成增加。在这里，我们在体外研究 CP 对婴儿肠道细菌的可能影响。通过在含有 CP 的培养基中培养来分析在婴儿肠道中占优势的几种细菌种类的生长。通过扫描透射电镜、膜染色、气相色谱和微生物燃料电池实验对作为敏感代表的普拉氏粪杆菌进行了详细分析。在所有测试的细菌中，几种双歧杆菌和F. prausnitzii的生长在 0.01 mg/ml CP 时减少，婴儿双歧杆菌完全停止生长。CP降低了F. prausnitzii的细胞包膜厚度，扰乱了细胞膜脂肪酸和参与电子传递的膜蛋白的功能。CP抑制双歧杆菌和粪杆菌的生长。这表明 IF 中脂肪的改变可能通过支持重要有益细菌在生命早期的定植，从而有利于配方奶喂养婴儿肠道微生物群的发育。需要未来的临床研究来证实这一点。
京公网安备 11010802027423号