Thirdhand cigarette smoke leads to age‐dependent and persistent alterations in the cecal microbiome of mice,MicrobiologyOpen

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Thirdhand cigarette smoke leads to age‐dependent and persistent alterations in the cecal microbiome of mice
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2021-05-15 , DOI: 10.1002/mbo3.1198
Li He _{1,

2} , Yan-Xia Zhou _{1,

3} , Yuqing Zhang _{1,

4} , Bo Hang ₁ , Hang Chang ₁ , Suzaynn F Schick ₅ , Susan E Celniker ₁ , Yankai Xia ₄ , Antoine M Snijders ₁ , Jian-Hua Mao ₁

Affiliation

The gut microbiome composition is influenced by many factors including environmental exposures. Here, we investigated the effect of thirdhand cigarette smoke (THS) and exposure age on gut microbiome diversity. C57BL/6 mice were exposed to THS at human exposure relevant levels for three weeks during three different life stages: postnatal (0–3 weeks of age), pubescent (4–7 weeks of age), and adult (9–12 weeks of age), respectively. Cecal microbiome profiles were assessed at 17 weeks of age by 16S rRNA gene sequencing. We found that age at THS exposure strongly influenced the cecal microbiome composition. Although postnatal THS exposure significantly influenced the microbial composition, pubescent and adulthood exposures only had minor effects. The microbiome of postnatally THS‐exposed mice significantly increased several degradation pathways that regulate glycolysis and pyruvate decarboxylation, and significantly decreased coenzyme A biosynthesis and pyrimidine deoxyribonucleoside salvage. Our results indicate that mouse postnatal development is particularly susceptible to persistent THS exposure effects on the gut microbiome.

中文翻译：

三手烟烟雾会导致小鼠盲肠微生物群发生年龄依赖性且持续的变化

肠道微生物组的组成受到许多因素的影响，包括环境暴露。在这里，我们研究了三手烟烟雾 (THS) 和暴露年龄对肠道微生物组多样性的影响。 C57BL/6 小鼠在三个不同的生命阶段暴露于人类暴露相关水平的 THS 三周：出生后（0-3 周龄）、青春期（4-7 周龄）和成年（9-12 周龄）年龄）分别。在 17 周龄时通过 16S rRNA 基因测序评估盲肠微生物组概况。我们发现，接触 THS 时的年龄强烈影响盲肠微生物组的组成。尽管出生后接触THS对微生物组成有显着影响，但青春期和成年期接触仅产生轻微影响。出生后暴露于 THS 的小鼠的微生物组显着增加了调节糖酵解和丙酮酸脱羧的多种降解途径，并显着减少了辅酶 A 的生物合成和嘧啶脱氧核糖核苷的回收。我们的结果表明，小鼠产后发育特别容易受到持续的 THS 暴露对肠道微生物组的影响。

更新日期：2021-05-17

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