Triticale (× Triticosecale Wittmack) is a commercial hybrid harboring wheat (Triticum sp.) and rye (Secale cereale L.) genomes. The limited genetic diversity of this crop resulted in the collapse of fungal disease resistance. Leaf rust disease, caused by Puccinia triticina Eriks., is reported to reduce the triticale yield significantly (more than 30%). There is a need to enlarge the genetic variability of this crop including leaf resistance genes. The main aim of this research was to evaluate the leaf rust resistance of the offspring of translocation lines of triticale carrying chromatin of Ae. tauschii and Ae. kotschyi. A reaction of seedlings of 200 plants of two triticale-Aegilops translocation lines (Bogo-2D^t.2R and Sekundo-2S^k.2R) was compared after inoculation with a natural mixture of P. triticina races, specific to triticale in controlled condition. Before inoculation, each plant was screened using molecular cytogenetics and molecular markers linked to leaf rust resistance genes. The presence of Aegilops chromosome segments was confirmed using genomic in situ hybridization (GISH). Lr39 and Lr54 leaf rust resistance genes were identified using Xgdm35 and S14 molecular markers, respectively. After inoculation, a significant improvement of resistance severity was observed in Sekundo-2S^k.2R in comparison with triticale cv. Sekundo plants. The resistance level of Bogo-2D^t.2R did not differ compared with triticale cv. Bogo plants. It was shown that Lr39 gene did not increase the leaf rust resistance level of triticale cv. Bogo.

黑小麦 (×  Triticosecale Wittmack) 是一种商业杂交种，含有小麦 ( Triticum sp.) 和黑麦 ( Secale graine L.) 基因组。这种作物有限的遗传多样性导致真菌抗病性的崩溃。据报道，由Puccinia triticina Eriks. 引起的叶锈病会显着降低黑小麦产量（超过 30%）。需要扩大这种作物的遗传变异性，包括叶片抗性基因。本研究的主要目的是评估携带Ae染色质的黑小麦易位系后代的叶锈病抗性。tauschii和Ae。科奇。两种小黑麦200株幼苗的反应-Aegilops易位系（Bogo-2D ^t .2R 和 Sekundo-2S ^k .2R）在接种后与受控条件下特定于黑小麦的P. triticina种族的天然混合物进行比较。在接种前，使用分子细胞遗传学和与叶锈病抗性基因相关的分子标记对每株植物进行筛选。使用基因组原位杂交 (GISH) 确认了Aegilops染色体片段的存在。使用Xgdm35和S14鉴定Lr39和Lr54叶锈病抗性基因分子标记，分别。^{接种后，Sekundo-2S k} .2R 与黑小麦 cv 相比，抗性严重程度显着提高。塞昆多植物。与黑小麦 cv 相比，Bogo-2D ^t .2R 的抗性水平没有差异。博戈植物。结果表明，Lr39基因不增加黑小麦的叶锈病抗性水平。博戈。