Paeoniae Radix Alba (PRA), an herbal drug produced from the root of Paeonia lactiflora Pall., is widely used in many herbal medicine prescriptions/preparations. Since the pharmacological effects of PRA come from multiple chemical components, it is important to establish a method for the determination of those components in PRA extracts with simple operation and low cost, which is more suitable to evaluate the quality of PRA extracts and optimize the extraction process. This work introduced the quantitative analysis of multicomponents with a single-marker (QAMS) method for the simultaneous determination of eleven bioactive components in PRA extracts, including gallic acid, oxypaeoniflorin, catechin, albiflorin, paeoniflorin, ethyl gallate, galloylpaeoniflorin, pentagalloylglucose, benzoic acid, benzoylpaeoniflorin, and paeonol. In the QAMS method established based on high performance liquid chromatography with diode array detection, only the reference substance of paeoniflorin was needed, and the other ten components were determined based on their relative correction factors (RCFs) to paeoniflorin. Moreover, the repeatability and robustness of the RCFs were studied with different column temperatures, detection wavelengths, flow rates, column types, and instruments. In method validation, good linearity (r > 0.999), stability, repeatability (RSD < 1.9%), and accuracy (recoveries within 96.1%–105.5%) were shown. Sample analyses showed that the QAMS method was consistent with the conventional external standard method. The established method provided a comprehensive, efficient, and low-cost tool for the routine quality evaluation of PRA extracts.

中文翻译：

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同时测定白e药草中11种成分的单一标准物质

白芍（PRA），从根产生的草药芍药颇尔广泛用于许多草药处方/制剂中。由于PRA的药理作用来自多种化学成分，因此建立一种测定操作简便，成本低廉的PRA提取物中这些成分的方法非常重要，它更适合评估PRA提取物的质量并优化提取工艺过程。这项工作介绍了使用单标记（QAMS）方法对多组分进行定量分析的方法，用于同时测定PRA提取物中的11种生物活性组分，其中包括没食子酸，羟eon药苷，儿茶素，白菊酯，pa药苷，没食子酸乙酯，没食子总酚苷，戊铝酰葡萄糖，苯甲酸，苯甲酰pa药苷和丹皮酚。在基于具有二极管阵列检测功能的高效液相色谱法建立的QAMS方法中，仅需要pa药苷的参比物质，其他十种成分是根据其对pa药苷的相对校正因子（RCF）确定的。此外，还使用不同的色谱柱温度，检测波长，流速，色谱柱类型和仪器对RCF的重复性和耐用性进行了研究。在方法验证中，良好的线性度（r  > 0.999），稳定性，可重复性（RSD <1.9％）和准确性（回收率在96.1％–105.5％之内）显示。样品分析表明，QAMS方法与常规外标方法一致。所建立的方法为PRA提取物的常规质量评估提供了一种全面，高效且低成本的工具。