The aim of this study was to investigate the in vivo and in vitro effects of dietary ω-6 and ω-3 polyunsaturated fatty acids (PUFAs) and their derivatives on the expression of TP53 and their relationship with cellular proliferation and death in a murine mammary adenocarcinoma model. BALB/c mice were divided in three diet groups: chia oil (ChO) rich in ω-3, corn oil (CO) rich in ω-6/ω-3 and safflower oil (SO) rich in ω-6 and subcutaneously inoculated with LMM3 mammary tumor cell line. Results demonstrated that diets with higher concentration of omega-6 PUFAs induced an increment of linoleic and arachidonic acid on tumor cell membranes increasing ROS liberation, 12(S)-HHT generation, TP53, Ki67 expression and cell proliferation. However, diets enriched with high content in omega-3 PUFAs induced higher tumor cell apoptosis and tumor infiltration of CD3+ lymphocytes, lowest cell viability and proliferation. Dietary omega-3 PUFAs nutritional intervention can be used as a potential preventative strategy to inhibit the molecular signaling pathways involved in the mammary tumor growth process as the TP53.

本研究的目的是研究膳食 ω-6 和 ω-3 多不饱和脂肪酸 (PUFA) 及其衍生物对小鼠乳腺中 TP53 表达的体内和体外影响及其与细胞增殖和死亡的关系腺癌模型。 BALB/c小鼠分为三个饮食组：富含ω-3的奇亚籽油（ChO）、富含ω-6/ω-3的玉米油（CO）和富含ω-6的红花油（SO）并皮下接种与LMM3乳腺肿瘤细胞系。结果表明，含有较高浓度 omega-6 PUFA 的饮食可诱导肿瘤细胞膜上的亚油酸和花生四烯酸增加，从而增加 ROS 释放、12(S)-HHT 生成、TP53、Ki67 表达和细胞增殖。然而，富含omega-3 PUFAs的饮食会诱导更高的肿瘤细胞凋亡和CD3+淋巴细胞的肿瘤浸润，以及最低的细胞活力和增殖。膳食 omega-3 PUFA 营养干预可作为一种潜在的预防策略，抑制 TP53 等参与乳腺肿瘤生长过程的分子信号通路。