Grey mould caused by Botrytis cinerea Pers. (teleomorph Botryotinia fuckeliana (de Bary) Whetzel) is one of the most destructive fungal diseases of Mediterranean crops. In Algeria, few studies have been made on the economic impact of this disease. Nevertheless, it is practically present in all tomato and strawberry greenhouses, as well as in prospected vineyards in the north and south of the country. The complexity of chemical control of this disease has led to search for Trichoderma strains that are effective in biological control. Fifteen isolates of Trichoderma spp. were obtained from vigorous and healthy plants (tomatoes, strawberries, and vines) rhizosphere, and from a commercial bio-compost (Bio-composte®), then identified as T. afroharzianum (four isolates), T. gamsii (four isolates), T. longibrachiatum (three isolates), T. atroviride (one isolate), T. brevicompactum (one isolate), T. breve (one isolate), and T. lixii (one isolate) on the basis of DNA sequence analysis of four genes (ITS, tef1, rpb2, and acl1). In vitro biocontrol tests revealed that four Algerian isolates of Trichoderma spp. (TAtC11, TGS7, TGS10, and TBS1) had a high antagonistic activity against B. cinerea, the mycelial growth has been reduced by 62 to 65% in dual-culture technique, by 62.31 to 64.49% in volatile compounds test, and a high inhibition of germling growth was recorded by TBS1 isolate with 90.68% in Culture filtrates test. Biocontrol tests carried out on tomato plants with T. brevicompactum (TBS1), T. atroviride (TAtC11), and T. lixii (TLiC8) against B. cinerea (BCT04) showed that TBS1 inoculation significantly reduced the incidence of disease by 64.43 and 51.35% in preventive and curative treatment, respectively. The present study revealed the first report of T. brevicompactum, T. breve, and T. lixii in Algeria, and it also contributes to the promotion of the use of native strains of Trichoderma in biological control leading to a better preservation of soil microbial diversity.

中文翻译：

---

从阿尔及利亚根际分离的三种木霉菌的首次报道以及短木霉菌对番茄灰霉病的高拮抗作用

由灰葡萄孢引起的灰霉病。（teleomorph Botryotinia fuckeliana（de Bary）Whetzel）是地中海作物最具破坏力的真菌病之一。在阿尔及利亚，很少有人对该疾病的经济影响进行研究。尽管如此，实际上它存在于所有番茄和草莓温室中，以及该国北部和南部的潜在葡萄园中。化学控制这种疾病的复杂性导致人们寻找在生物控制中有效的木霉菌株。十五种木霉属菌株。得自有力和健康的植物（番茄，草莓和葡萄藤）根际，以及商业化的生物堆肥（Bio-composte®），然后被鉴定为非洲山毛榉（T. afroharzianum）（四种分离株），gamsii gamsii（四种分离株）， T. longibrachiatum（三个分离株），T. 根据四个基因（ITS，tef1，rpb2和acl1）的DNA序列分析确定atroviride（一种分离物），br.T。brevicompactum（一种分离物），breve（一种分离物）和T. lixii（一种分离物） 。体外生物防治测试显示，有四个木霉属的阿尔及利亚分离株。（TAtC11，TGS7，TGS10和TBS1）对灰葡萄孢具有很高的拮抗活性，在双重培养技术中，菌丝体生长降低了62％至65％，在挥发性化合物测试中降低了62.31％至64.49％，并且在培养物滤液试验中，TBS1分离物记录了胚芽生长的抑制作用，分离率为90.68％。对番茄芽孢杆菌（BCT04）进行了番茄短杆菌（TBS1），阿魏病毒（Tttrotroide）（TAtC11）和利氏螺旋体（TLiC8）番茄植株的生防测试，结果表明，接种TBS1可以显着降低疾病发病率64.43和51。 。预防和治疗分别占35％。本研究揭示了阿尔及利亚短杆菌，短杆菌和利克斯氏杆菌的首次报道，它也有助于促进木霉天然菌株在生物防治中的应用，从而更好地保存土壤微生物多样性。