Cold atmospheric plasma (CAP) is a potential anticancer therapy. CAP has cytotoxic effects when applied either directly to cancer cell cultures or indirectly through plasma-conditioned liquids. This protocol describes how to treat adherent cultures of human cancer cell lines with CAP or plasma-conditioned medium and determine cell viability following treatment. The protocol also includes details on how to quantify the reactive oxygen and nitrogen species present in medium following CAP treatment, using chemical probes using UV-visible or fluorescence spectroscopy. CAP treatment takes ~30 min, and 3 h are required to complete quantification of reactive oxygen and nitrogen species. By providing a standardized protocol for evaluation of the effects of CAP and plasma-conditioned medium, we hope to facilitate the comparison and interpretation of results seen across different laboratories.

冷大气等离子体（CAP）是一种潜在的抗癌疗法。当直接应用于癌细胞培养物或通过血浆条件液体间接应用时，CAP 具有细胞毒性作用。该协议描述了如何用 CAP 或血浆条件培养基处理人类癌细胞系的贴壁培养物，并确定治疗后的细胞活力。该协议还包括有关如何使用使用紫外可见或荧光光谱的化学探针来量化 CAP 处理后介质中存在的活性氧和氮物质的详细信息。CAP 处理需要约 30 分钟，并且需要 3 小时来完成活性氧和氮物质的定量。通过提供标准化方案来评估 CAP 和血浆条件培养基的效果，