The pretreatment of wheat straw has been recognized to be an essential step prior to anaerobic digestion, owing to the high abundance of lignocellulosic materials. In order to choose economical and effective techniques for the disposal of wheat straw, effects of five pretreatment methods including acid, alkali, co-pretreatment of acid and alkali, CaO₂, and liquid digestate of municipal sewage sludge on anaerobic digestion of wheat straw were investigated by analyzing biogas production and organic matter degradation in the study. The results showed that among these pretreatment methods, the methane yield was highest in the liquid digestate pretreated-wheat straw with 112.6 mL g_TS⁻¹, followed by the acid, alkali, and CaO₂ pretreatments, and the lowest was observed in the co-pretreatment of acid and alkali. Illumina MiSeq sequencing of the microbial communities in the anaerobic digesters revealed that the genera Ruminiclostridium including Ruminiclostridium and Ruminiclostridium 1, Hydrogenispora, and Capriciproducens were the main hydrolytic bacteria, acidogenic bacteria, and acetogenic bacteria, respectively, in the anaerobic digesters. Capriciproducens and Hydrogenispora dominated in the first and the later stages, respectively, in the anaerobic digesters, which could work as indicators of the anaerobic co-digestion stage of sludge and wheat straw. The total solid and SO₄²⁻-S contents of the solid digestate and the NH₄⁺-N concentration of the liquid digestate had a significant influence on the microbial community in the digesters. These findings indicated that liquid digestate pretreatment was a potential option to improve the anaerobic digestion of wheat straw, due to the low cost without additional chemical agents.

由于木质纤维素材料的丰度高，小麦秸秆的预处理已被认为是厌氧消化之前必不可少的步骤。为选择经济有效的小麦秸秆处理工艺，研究了酸、碱、酸碱协同预处理、CaO ₂、城市污水污泥消化液5种预处理方式对麦秸厌氧消化的影响。通过分析研究中的沼气生产和有机物降解进行调查。结果表明，在这些预处理方法中，液体消化液预处理小麦秸秆的甲烷产率最高，为 112.6 mL g _TS ^-1，其次是酸、碱和 CaO ₂预处理，酸碱联合预处理最低。在厌氧消化的微生物群落的Illumina的MiSeq测序，发现该属Ruminiclostridium包括Ruminiclostridium和Ruminiclostridium 1，Hydrogenispora和Capriciproducens是主要的水解细菌，产酸细菌和产乙酸细菌，分别在厌氧消化器。Capriciproducens和Hydrogenispora分别在厌氧消化器的第一阶段和后期占主导地位，可以作为污泥和麦秸共消化厌氧阶段的指标。总固体和 SO固体消化物中的₄ ^2- -S 含量和液体消化物中的 NH ₄ ⁺ -N 浓度对消化池中的微生物群落有显着影响。这些发现表明，液体消化物预处理是改善小麦秸秆厌氧消化的潜在选择，因为成本低，无需额外的化学试剂。