Background & objectives

Dodonaea viscosa is one of the natural medications utilized in Saudi Arabia for treatment of several diseases and disorders. Prior investigations have revealed its anti-inflammatory and antioxidant properties. In the present study, the chloroform and methanolic extracts of Dodonaea viscosa were formulated as ointments using the emulsifying base and its wound healing property was determined in animal models.

Materials and methods

The dried leaves of Dodonaea viscosa was macerated in methanol and chloroform solvents to obtain respective solvent extracts. Both the extracts were subjected for phytochemical investigation. A 10% w/w herbal ointment of the extracts were formulated and their physicochemical properties; color and odor, loss on drying, pH, and spreadability were evaluated. Further, both the formulations were tested for stability studies. The wound healing activity of individual herbal formulations were studied in Sprague Dawley rats using incision and excision wound models. The application of ointment was done topically once every day up to the end of the healing of the wound. The epithelization period in the excision wound model and tensile strength in the incision wound model were assessed to determine the wound-healing effects.

Results

Methanolic and chloroform extracts of Dodonaea viscosa leaves have flavonoids and saponins in addition to other phytochemicals. All the evaluated physicochemical properties of both the formulations were within ideal cutoff points and they remained stable. In comparison with control and base groups, methanolic extract formulation took significantly (p < 0.001) less time for epithelization of the excision wound. A significant (p < 0.001) increase in the tensile strength was observed in the incision wound model in group of animals treated with either methanolic or chloroform extract formulations when compared with other groups.

Interpretation & conclusion

It was evident from the results that the extracts had the potential for use as wound healing agents. The wound healing potency of the extracts could be an element of either the individual bioactive constituents or synergistic impact of number of active molecules present in the extract.

中文翻译：

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十二指肠提取物制剂在实验动物中的伤口愈合潜力

背景与目标

Dodonaea viscosa是沙特阿拉伯用于治疗多种疾病的天然药物之一。先前的研究已经揭示了它的抗炎和抗氧化特性。在本研究中，使用乳化基质将十二指肠的氯仿和甲醇提取物配制成软膏，并在动物模型中确定其伤口愈合特性。

材料和方法

十二指肠干叶将其在甲醇和氯仿溶剂中浸渍，以获得相应的溶剂提取物。两种提取物都进行了植物化学研究。配制了提取物的10％w / w草药软膏，并对其理化性质进行了分析。评估颜色和气味，干燥失重，pH值和铺展性。此外，两种制剂都经过了稳定性研究测试。使用切口和切除伤口模型在Sprague Dawley大鼠中研究了各个草药制剂的伤口愈合活性。直到伤口愈合结束为止，每天都要局部涂抹一次软膏。评估切除伤口模型中的上皮化时期和切口伤口模型中的抗张强度，以确定伤口的愈合效果。

结果

远藤叶片的甲醇和氯仿提取物除其他植物化学物质外还含有类黄酮和皂苷。两种制剂的所有评估的理化性质均在理想的临界点内，并且它们保持稳定。与对照组和基础组相比，甲醇提取物制剂 在切除伤口上皮化上花费的时间明显减少（p <0.001）。 与其他组相比，在用甲醇或氯仿提取物制剂治疗的动物组中，在切口伤口模型中观察到的拉伸强度显着提高（p <0.001）。

解释与结论

结果表明，提取物具有用作伤口愈合剂的潜力。提取物的伤口愈合能力可以是单个生物活性成分的组成部分，也可以是提取物中存在的许多活性分子的协同影响的要素。