A diploid potato (Solanum tuberosum L.) recombinant inbred line population was derived from a cross between Solanum chacoense inbred line M6 and S. chacoense accession USDA8380-1 (80-1) to identify loci associated with self-compatibility and Colorado potato beetle resistance. Individuals from the F₄ and F₅ generations were genotyped on the Illumina Infinium V3 22K Single Nucleotide Polymorphism (SNP) Array and a genetic map constructed. All F₅ individuals contain at least one copy of the dominant S-locus inhibitor (Sli) haplotype; however, not all F₅ individuals set fruit. Pollen tubes reached the ovaries of both self-fruitful and self-unfruitful plants, indicating that the presence of the dominant Sli allele is not sufficient for selfed fruit and seed production. Loci on chromosomes 3, 5, 6, and 12 were identified as novel targets for self-fertility improvement. Evaluation of fruit and seed set upon selfing in the F₄ generation over two greenhouse seasons revealed environmental influence on self-fertility. Loci exhibiting residual heterozygosity were found on all chromosomes except chromosomes 3 and 11 in F₅ inbreds, but none of the measured self-fertility traits were correlated with the level of heterozygosity based on SNP genotyping. Four SNPs on chromosome 2 between 22,151,711 and 22,381,719 bp were associated with foliar leptine glycoalkaloid synthesis and Colorado potato beetle resistance in the recombinant inbred line population. Robust inbred lines carrying Colorado potato beetle resistance were developed without field selection during the inbreeding process and beetle resistance was introgressed into diploid breeding lines.

中文翻译：

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在二倍体 Solanum chacoense 重组近交系种群中的自我生育能力和对科罗拉多马铃薯甲虫 (Leptinotarsa decemlineata) 的抗性

二倍体马铃薯 ( Solanum tuberosum L.) 重组自交系群体来自于Solanum chacoense自交系 M6 和S. chacoense种质 USDA8380-1 (80-1)之间的杂交，以鉴定与自交亲和性和科罗拉多马铃薯甲虫抗性相关的位点. 来自 F ₄和 F ₅代的个体在 Illumina Infinium V3 22K 单核苷酸多态性 (SNP) 阵列上进行基因分型，并构建了遗传图谱。所有 F ₅个体都包含至少一个显性S基因座抑制剂 ( Sli ) 单倍型的拷贝；然而，并非所有 F ₅个人结出果实。花粉管到达自花和自花植物的子房，表明显性Sli等位基因的存在不足以产生自交果实和种子。染色体 3、5、6 和 12 上的基因座被确定为改善自我生育能力的新目标。对 F ₄代自交后两个温室季节的果实和种子的评估揭示了环境对自育能力的影响。在 F _{5 中}除 3 号和 11 号染色体外，所有染色体上均发现表现出残留杂合性的位点近交系，但没有一个测量的自育性状与基于 SNP 基因分型的杂合性水平相关。2 号染色体上 22,151,711 和 22,381,719 bp 之间的四个 SNP 与重组自交系群体中叶面瘦素糖苷生物碱合成和科罗拉多马铃薯甲虫抗性有关。在近交过程中，在没有田间选择的情况下开发了携带科罗拉多马铃薯甲虫抗性的稳健近交系，并将甲虫抗性渗入二倍体育种系。