Bomb-produced radiocarbon (¹⁴C) was used to validate age estimates of Greenland halibut (Reinhardtius hippoglossoides) using a stained otolith cross-section method. The Δ¹⁴C in eastern Bering Sea (EBS) Greenland halibut otoliths was compared to both EBS and Gulf of Alaska (GOA) Pacific halibut (Hippoglossoides stenolepis) otolith reference chronologies to evaluate which reference chronology was most suitable, and to quantitatively estimate age determination bias. Using Bayesian analysis and a coupled-function model, the Δ¹⁴C in the Greenland halibut showed greatest similarities to the Δ¹⁴C in the GOA reference chronology. Although the model indicated under ageing, the bias was not large. Assigning an age less than the true age by more than a one year is about 73%, and less than the true age by more than 2 years is only about 25%. When considering the age at which Greenland halibut is only 7.5% of its maximum longevity (50+ years) and that the probability of underageing by 3 years being less than 5%, it is likely that between-age-reader variation will cancel out any systematic bias that exists in the age determination protocols. Prior to the use of stained cross-sections the maximum age was 38 years, now a maximum age of 53 years is supported.

炸弹产生的放射性碳（¹⁴ C）用于使用染色的耳石横截面方法验证格陵兰大比目鱼（Reinhardtius hippoglossoides）的年龄估计。的Δ ¹⁴在白令海东海（EBS）格陵兰Ç比目鱼耳石物相比，无论EBS和阿拉斯加（GOA）太平洋大比目鱼（湾Hippoglossoides stenolepis）耳石参考年表评估哪些参考年表是最合适的，并定量地估计年龄测定偏见。使用贝叶斯分析和耦合功能模型中，Δ ¹⁴在格陵兰Ç大比目鱼表明最大相似性的Δ ¹⁴GOA参考年表中的C。尽管模型显示老化，但偏差并不大。将年龄指定为比真实年龄小一年以上大约为73％，而将其指定为小于真实年龄2年以上大约只有25％。考虑到格陵兰岛大比目鱼的年龄仅为其最大寿命（超过50年）的7.5％，而3岁以下的未成年人几率小于5％，年龄不同的读者之间的差异很可能会抵消年龄确定协议中存在系统性偏见。在使用染色横截面之前，最大年龄为38岁，现在支持最大年龄为53岁。