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Cell stiffness predicts cancer cell sensitivity to ultrasound as a selective superficial cancer therapy
Bioengineering & Translational Medicine ( IF 6.1 ) Pub Date : 2021-05-13 , DOI: 10.1002/btm2.10226 Eden Bergman 1 , Riki Goldbart 1 , Tamar Traitel 1 , Eliz Amar-Lewis 1 , Jonathan Zorea 2 , Ksenia Yegodayev 2 , Irit Alon 3, 4 , Sanela Rankovic 5 , Yuval Krieger 6 , Itay Rousso 5 , Moshe Elkabets 2 , Joseph Kost 1
Bioengineering & Translational Medicine ( IF 6.1 ) Pub Date : 2021-05-13 , DOI: 10.1002/btm2.10226 Eden Bergman 1 , Riki Goldbart 1 , Tamar Traitel 1 , Eliz Amar-Lewis 1 , Jonathan Zorea 2 , Ksenia Yegodayev 2 , Irit Alon 3, 4 , Sanela Rankovic 5 , Yuval Krieger 6 , Itay Rousso 5 , Moshe Elkabets 2 , Joseph Kost 1
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We hypothesize that the biomechanical properties of cells can predict their viability, with Young's modulus representing the former and cell sensitivity to ultrasound representing the latter. Using atomic force microscopy, we show that the Young's modulus stiffness measure is significantly lower for superficial cancer cells (squamous cell carcinomas and melanoma) compared with noncancerous keratinocyte cells. In vitro findings reveal a significant difference between cancerous and noncancerous cell viability at the four ultrasound energy levels evaluated, with different cell lines exhibiting different sensitivities to the same ultrasound intensity. Young's modulus correlates with cell viability (R2 = 0.93), indicating that this single biomechanical property can predict cell sensitivity to ultrasound treatment. In mice, repeated ultrasound treatment inhibits tumor growth without damaging healthy skin tissue. Histopathological tumor analysis indicates ultrasound-induced focal necrosis at the treatment site. Our findings provide a strong rationale for developing ultrasound as a noninvasive selective treatment for superficial cancers.
中文翻译：
细胞硬度预测癌细胞对超声波作为选择性浅表癌症治疗的敏感性
我们假设细胞的生物力学特性可以预测其活力，杨氏模量代表前者，细胞对超声的敏感性代表后者。使用原子力显微镜，我们发现与非癌性角质形成细胞相比，浅表癌细胞（鳞状细胞癌和黑色素瘤）的杨氏模量刚度测量值显着较低。体外研究结果表明，在所评估的四种超声能量水平下，癌细胞和非癌细胞的活力存在显着差异，不同的细胞系对相同的超声强度表现出不同的敏感性。杨氏模量与细胞活力相关（ R 2 = 0.93），表明这种单一的生物力学特性可以预测细胞对超声治疗的敏感性。在小鼠中，重复的超声波治疗可以抑制肿瘤生长，而不损害健康的皮肤组织。组织病理学肿瘤分析表明治疗部位存在超声诱导的局灶性坏死。我们的研究结果为开发超声波作为浅表癌症的非侵入性选择性治疗方法提供了强有力的理由。
更新日期：2021-05-13
中文翻译：
细胞硬度预测癌细胞对超声波作为选择性浅表癌症治疗的敏感性
我们假设细胞的生物力学特性可以预测其活力，杨氏模量代表前者，细胞对超声的敏感性代表后者。使用原子力显微镜，我们发现与非癌性角质形成细胞相比，浅表癌细胞（鳞状细胞癌和黑色素瘤）的杨氏模量刚度测量值显着较低。体外研究结果表明，在所评估的四种超声能量水平下，癌细胞和非癌细胞的活力存在显着差异，不同的细胞系对相同的超声强度表现出不同的敏感性。杨氏模量与细胞活力相关（ R 2 = 0.93），表明这种单一的生物力学特性可以预测细胞对超声治疗的敏感性。在小鼠中，重复的超声波治疗可以抑制肿瘤生长，而不损害健康的皮肤组织。组织病理学肿瘤分析表明治疗部位存在超声诱导的局灶性坏死。我们的研究结果为开发超声波作为浅表癌症的非侵入性选择性治疗方法提供了强有力的理由。
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