Abstract—

The somatic isoform of the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) is involved in such crucial pathways for cancer cells development as induction of apoptosis and regulation of glycolysis. At the same time, sperm-specific isoform (GAPDHS) does not exhibit all the same functions as the somatic enzyme. The expression of sperm-specific GAPDH without N-terminal domain in some melanoma cells along with somatic isoenzyme, shown in our previous work, has led to the proposal of this unusual enzyme’s possible role in regulation of cancer cells glycolysis. In this study we have tested production of GAPDHS in 13 additional melanoma cell lines by immunoblotting. We have also gathered data on energy metabolism in 5 selected cell lines by evaluating glucose uptake and lactate production in different conditions. We have demonstrated that in standard cultivation media glucose uptake by MelP cells, producing substantial amounts of GAPDHS protein was higher than in MelKor cells, producing lesser amounts of GAPDHS. All other analyzed cell lines that do not produce GAPDHS (MelMS, MelSi and Malme3M) had even a lower glucose uptake rate.

中文翻译：

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黑色素瘤细胞中精子特异性甘油醛-3-磷酸脱氢酶的表达改变其能量代谢

摘要—

糖酵解酶3-磷酸甘油醛脱氢酶（GAPDH; EC 1.2.1.12）的体细胞同工型参与了癌细胞发展的关键途径，如诱导凋亡和调节糖酵解。同时，精子特异性同工型（GAPDHS）并没有表现出与体酶相同的所有功能。我们先前的研究表明，某些黑素瘤细胞中无N末端结构域的精子特异性GAPDH连同体细胞同工酶一起表达，提示了这种异常酶在调节癌细胞糖酵解中的可能作用。在这项研究中，我们已经通过免疫印迹测试了另外13种黑色素瘤细胞系中GAPDHS的产生。我们还通过评估在不同条件下的葡萄糖摄取和乳酸生成，收集了5种选定细胞系中能量代谢的数据。我们已经证明，在标准培养基中，MelP细胞对葡萄糖的吸收产生大量的GAPDHS蛋白要高于MelKor细胞，产生较少的GAPDHS蛋白。所有其他不产生GAPDHS的分析细胞系（MelMS，MelSi和Malme3M）甚至具有更低的葡萄糖摄取率。