In this study, the effect of rutin on fatty acid metabolism and antioxidant activity were evaluated. We found that the antioxidant capacity of rutin-treated Caenorhabditis elegans was enhanced but the triglyceride content was significantly reduced. The reduction of fat accumulation by rutin was also confirmed by Oil Red O staining. RNA-seq analysis indicated that rutin significantly regulated the expression of seven genes related to lipid metabolism in C. elegans. Among the seven genes, acox-1.3, stdh-3, and fat-7 were associated with fatty acid metabolism. Rutin significantly reduced fat accumulation in both fat-6 and fat-7 mutant strains but did not affect the fat storage of fat-6/fat-7 double mutant, which indicated that the impact of rutin on fat storage depended on fat-6 and fat-7. These findings indicated that rutin reduced fat storage depending on the regulation of lipid metabolism-related genes expression and thereby regulating the biosynthesis of the corresponding unsaturated fatty acid.

中文翻译：

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芦丁对秀丽隐杆线虫的抗氧化和降脂作用

在这项研究中，评估了芦丁对脂肪酸代谢和抗氧化活性的影响。我们发现芦丁处理的秀丽隐杆线虫的抗氧化能力增强，但甘油三酯含量显着降低。油红 O 染色也证实了芦丁对脂肪积累的减少。RNA-seq 分析表明，芦丁显着调节了秀丽隐杆线虫中与脂质代谢相关的 7 个基因的表达。7个基因中，acox-1.3、stdh-3、fat-7与脂肪酸代谢相关。芦丁显着减少fat -6和fat-7中的脂肪积累突变菌株但不影响fat-6 / fat-7双突变体的脂肪储存，这表明芦丁对脂肪储存的影响取决于fat-6和fat-7。这些发现表明，芦丁通过调节脂质代谢相关基因的表达来减少脂肪储存，从而调节相应不饱和脂肪酸的生物合成。