Ammonia is the mainly environmental pollutant in the field of aquaculture in association with negative impact on the healthy cultivation of common carp. Gene expression profiles in the hepatopancreas of common carp treated with ammonia stress using an RNA-seq technique, and

22,431,825 and 23,592,342 clean reads from the control and treatment groups by a 96-h ammonia challenge (0.85 mg/L unionized ammonia, NH3) are obtained. Total amount of 3367 unigenes are identified as differentially expressed genes (DEGs) related to ammonia stress. The 1724 DEGs are down-regulated and 1643 DEGs are upregulated. The metabolic pathways of these DEGs are classified into 49 different terms by KEGG pathway analysis. Protein processing in the endoplasmic reticulum (ko04141) pathway is a significantly enriched pathway of DEGs. Many DEGs are enriched in the endoplasmic reticulum stress (ERS) pathway and unfolded protein response (UPR) signal pathway in the ko04141 pathway. ERS and UPR pathway are involved in the response of hepatopancreas cells by ammonia stress. The RT-PCR and transcriptomic results indicate that PERK- eIF2α and IRE1-xbp1 pathways take participate in the response to ERS in hepatopancreas cells induced by ammonia stress. The TUNEL assays results confirmed that ammonia stress induced apoptosis in hepatopancreatic cells. Furthermore, apoptosis of hepatopancreas cells under ammonia-stress is definitely induced by ERS through the PERK-eIF2α-CHOP and IRE1-XBP1-CHOP signaling pathways.

氨是水产养殖领域的主要环境污染物，对鲤鱼的健康养殖产生负面影响。使用RNA-seq技术处理氨水胁迫的鲤鱼肝胰腺中的基因表达谱，以及

从对照组和处理组通过96 h氨激发（0.85 mg / L工会化氨，NH3）获得了22,431,825和23,592,342的干净读数。总计3367个单基因被鉴定为与氨胁迫相关的差异表达基因（DEG）。下调了1724个DEG，上调了1643个DEG。通过KEGG途径分析，将这些DEG的代谢途径分为49个不同的术语。内质网（ko04141）途径中的蛋白质加工是DEG的显着富集途径。在ko04141途径中，许多DEG富含内质网应激（ERS）途径和未折叠的蛋白质反应（UPR）信号途径。ERS和UPR途径参与氨应激对肝胰腺细胞的反应。RT-PCR和转录组结果表明，PERK-eIF2α和IRE1-xbp1途径参与了氨胁迫诱导的肝胰腺细胞对ERS的反应。TUNEL测定结果证实氨胁迫诱导了肝胰腺细胞凋亡。此外，ERS通过PERK-eIF2α-CHOP和IRE1-XBP1-CHOP信号通路肯定诱导了氨胁迫下肝胰腺细胞的凋亡。