Abstract

In our ongoing search for new secondary metabolites from fungi, a basidiomycete fungus Irpex consors was selected for mycochemical investigation, and three new zwitterionic alkaloids (1-3) and five known compounds (4-8) were isolated from the culture broth (16 l) of I. consors. The culture filtrate was fractionated by a series of column chromatography including Diaion HP-20, silica gel, and Sephadex LH-20, Sep-Pak C₁₈ cartridge, medium pressure liquid chromatography (MPLC), and high pressure liquid chromatography (HPLC) to yield eight compounds (1-8). The structures of the isolated compounds were elucidated by the interpretation of nuclear magnetic resonance (NMR) spectra and high-resolution mass spectrometry (HR-MS). Their antioxidant and antibacterial activities were examined. The zwitterionic structures of three new sesquiterpene alkaloids (1-3) were determined together with five known compounds identified as stereumamide E (4), stereumamide G (5), stereumamide H (6), stereumamide D (7), and sterostrein H (8). This is the first report of the zwitterionic alkaloids in the culture broth of I. consors. Three new zwitterionic alkaloids were named as consoramides A–C (1-3).

摘要

在我们不断从真菌中寻找新的次生代谢物的过程中，我们选择了一种担子菌真菌Irpex consors进行真菌化学研究，并从培养液（16 升）中分离出了三种新的两性离子生物碱（1 - 3）和五种已知化合物（4 - 8）。 ) 的I. consors。培养物滤液经 Diaion HP-20、硅胶、Sephadex LH-20、Sep-Pak C _{18 小}柱、中压液相色谱 (MPLC) 和高压液相色谱 (HPLC) 等一系列柱层析分离，产生八种化合物 ( 1 - 8）。通过核磁共振 (NMR) 光谱和高分辨率质谱 (HR-MS) 的解释阐明了分离化合物的结构。检查了它们的抗氧化和抗菌活性。确定了三种新型倍半萜生物碱 ( 1 - 3 )的两性离子结构以及五种已知化合物，分别为立体酰胺 E ( 4 )、立体酰胺 G ( 5 )、立体酰胺 H ( 6 )、立体酰胺 D ( 7 ) 和立体甾醇 H ( 8）。这是第一次报道I. consors培养液中的两性离子生物碱。三种新的两性离子生物碱被命名为 consoramides A-C (1 - 3 )。