Bacterial effector molecules are crucial infectious agents that can cause pathogenesis. In the present study, the pathogenesis of toxic Salmonella enterica serovar Typhi (S. Typhi) proteins on the model host Caenorhabditis elegans was investigated by exploring the host’s regulatory proteins during infection through the quantitative proteomics approach. Extracted host proteins were analyzed using two-dimensional gel electrophoresis (2D-GE) and differentially regulated proteins were identified using MALDI TOF/TOF/MS analysis. Of the 150 regulated proteins identified, 95 were downregulated while 55 were upregulated. The interaction network of regulated proteins was predicted using the STRING tool. Most downregulated proteins were involved in muscle contraction, locomotion, energy hydrolysis, lipid synthesis, serine/threonine kinase activity, oxidoreductase activity, and protein unfolding. Upregulated proteins were involved in oxidative stress pathways. Hence, cellular stress generated by S. Typhi proteins in the model host was determined using lipid peroxidation as well as oxidant and antioxidant assays. In addition, candidate proteins identified via extract analysis were validated by western blotting, and the roles of several crucial molecules were analyzed in vivo using transgenic strains (myo-2 and col-19) and mutant (ogt-1) of C. elegans. To the best of our knowledge, this is the first study to report protein regulation in host C. elegans exposed to toxic S. Typhi proteins. It highlights the significance of p38 MAPK and JNK immune pathways.

细菌效应分子是可引起发病机制的重要感染因子。在本研究中，毒性肠杆菌血清型伤寒沙门氏菌 ( S. Typhi) 蛋白在模型宿主秀丽隐杆线虫上的发病机制通过定量蛋白质组学方法探索宿主在感染过程中的调节蛋白进行了研究。使用二维凝胶电泳 (2D-GE) 分析提取的宿主蛋白，并使用 MALDI TOF/TOF/MS 分析鉴定差异调节蛋白。在确定的 150 种受调节蛋白质中，95 种被下调，55 种被上调。使用 STRING 工具预测受调节蛋白质的相互作用网络。大多数下调的蛋白质参与肌肉收缩、运动、能量水解、脂质合成、丝氨酸/苏氨酸激酶活性、氧化还原酶活性和蛋白质展开。上调的蛋白质参与氧化应激途径。因此， S产生的细胞应激. 使用脂质过氧化作用以及氧化剂和抗氧化剂测定法确定模型宿主中的伤寒蛋白。此外，通过提取物分析鉴定的候选蛋白质通过蛋白质印迹法进行了验证，并使用秀丽隐杆线虫的转基因菌株（ myo -2 和col - 19）和突变体 ( ogt-1)对几种关键分子的作用进行了体内分析。据我们所知，这是第一项报告暴露于有毒S的宿主秀丽隐杆线虫中蛋白质调节的研究。伤寒蛋白。它突出了 p38 MAPK 和 JNK 免疫通路的重要性。