Ruminal microbiota changes frequently with high grain diets and the occurrence of subacute ruminal acidosis (SARA). A grain-induced goat model of SARA, with durations of a significant decrease in the rumen pH value to less than 5.6 and an increase in the rumen lipopolysaccharides concentration, is constructed for real-time monitoring of bacteria alteration. Using 16 S rRNA gene sequencing, significant bacterial differences between goats from the SARA and healthy groups are identified at every hour for six continuous hours after feeding. Moreover, 29 common differential genera between two groups over 6 h after feeding are all related to the altered pH and lipopolysaccharides. Transplanting the microbiota from donor goats with SARA could induce colonic inflammation in antibiotic-pretreated mice. Overall, significant differences in the bacterial community and rumen fermentation pattern between the healthy and SARA dairy goats are real-time monitored, and then tested using ruminal microbe transplantation to antibiotic-treated mice.

瘤胃微生物群随着高谷物日粮和亚急性瘤胃酸中毒（SARA）的发生而频繁变化。为了实时监测细菌的变化，构建了一种谷物诱导的 SARA 山羊模型，其瘤胃 pH 值显着降低至低于 5.6 的持续时间和瘤胃脂多糖浓度的增加。使用 16 S rRNA 基因测序，在喂食后连续 6 小时内每隔一小时就确定来自 SARA 和健康组的山羊之间的显着细菌差异。此外，在喂食后 6 小时内，两组之间的 29 个常见差异属都与 pH 值和脂多糖的改变有关。用 SARA 移植供体山羊的微生物群可以诱导抗生素预处理小鼠的结肠炎症。全面的，