The proteoglycanase (PGase) gene secreted of Burkholderia gladioli pv. alliicola was cloned to examine the correlation between the properties of this enzyme gene and pathogenicity. Recombinant clones of about 20 kb that produce PGase were separated from the genomic library of B. alliicola CH1. And it was shown that PGase gene was located within a fragment of about 2.6 kb of ApaI-XhoI restriction enzyme. The pehA gene indicated 79% homogeneity with the phg-A gene produced by Burkholderia cepacia (B. cepacia) plasmid pPEC320. PGase takes a structure in which α-helix connects the outer part of β-barrel with β-sheet, a common characteristic of enzyme structures that decomposes plant cell walls. The pehA gene macerated as if it were gnawing at the onion tissue.

剑兰伯克霍尔德菌分泌的蛋白聚糖酶 (PGase) 基因。克隆alliicola以检查该酶基因的特性与致病性之间的相关性。从大蒜芽孢杆菌CH1的基因组文库中分离出约 20 kb 的重组克隆，其产生 PGase 。结果表明PGase基因位于ApaI-XhoI限制酶约2.6kb的片段内。的PEHA基因表明79％均一性与PHG-A通过产生基因洋葱伯克霍尔德菌（洋葱伯克霍尔德菌）质粒pPEC320。PGase采用α-螺旋将β-桶的外部与β连接起来的结构-sheet，分解植物细胞壁的酶结构的共同特征。该六胺基因浸软，就好像它是在洋葱组织啃。